Zhang Lixia, Chen Qiang, Hu Jing, Chen Yue, Liu Chenglong, Xu Changshui
Jiaxing Maternity and Child Health Care Hospital, Jianxin 314050, China.
Department of Physiology, Basic Medical College, Nanchang University, Nanchang 330006, China.
Biomed Res Int. 2016;2016:5631935. doi: 10.1155/2016/5631935. Epub 2016 Jun 20.
CSCC is a systemic disease involving polygenic alteration and multiple steps, and HIF and VEGF are closely associated with tumorigenesis. Specimens surgically resected from 64 cases of CSCC and 22 cases of normal cervical tissue were selected randomly to detect the expression of HIF-2α and VEGF in CSCC for exploring their clinical significance; information regarding the age, lymph node metastasis, and FIGO staging were collected as well; expression of HIF-2α and VEGF was detected by qPCR and immunohistochemistry. We found that the expression of HIF-2α and VEGF mRNA in CSCC was significantly higher than that of normal cervical tissues and showed a positive correlation between them. The positive rates of HIF-2α and VEGF protein expression in CSCC and normal cervical tissues were 93.8% and 18.2%, respectively, with correlation between them. The expression of both HIF-2α and VEGF mRNA did not relate closely to age but the FIGO staging and lymph node metastasis. Compared with the counterpart control group, CSCC tissues with high FIGO staging and lymph node metastasis had a higher level of HIF-2α and VEGF mRNA expression. So, HIF-2α and VEGF were overexpressed in CSCC, which has a great clinical significance for its diagnosis.
皮肤鳞状细胞癌（CSCC）是一种涉及多基因改变和多个步骤的系统性疾病，缺氧诱导因子（HIF）和血管内皮生长因子（VEGF）与肿瘤发生密切相关。随机选取64例CSCC手术切除标本及22例正常宫颈组织标本，检测CSCC中HIF-2α和VEGF的表达，以探讨其临床意义；同时收集患者年龄、淋巴结转移及国际妇产科联盟（FIGO）分期等信息；采用实时荧光定量聚合酶链反应（qPCR）和免疫组织化学法检测HIF-2α和VEGF的表达。结果发现，CSCC中HIF-2α和VEGF mRNA的表达明显高于正常宫颈组织，且二者呈正相关。CSCC和正常宫颈组织中HIF-2α和VEGF蛋白表达的阳性率分别为93.8%和18.2%，二者具有相关性。HIF-2α和VEGF mRNA的表达与年龄无密切关系，但与FIGO分期和淋巴结转移有关。与相应对照组相比，FIGO分期高及有淋巴结转移的CSCC组织中HIF-2α和VEGF mRNA表达水平更高。因此，HIF-2α和VEGF在CSCC中过度表达，对其诊断具有重要临床意义。
