Kubacki S J, Havery D C, Fazio T
Z Gesamte Hyg. 1989 Feb;35(2):98-100.
An attempt has been made to develop conditions for the analysis of some N-nitrosamino acids and N-nitrosodipeptides N-thermal in proline by means of high performance liquid chromatography with thermal energy detector (HPLC-TEA). Two HPLC columns have been tested; cyano and amine chemically bonded columns. Although both of them give positive results baseline separation all of the compounds investigated takes place only on the amine column. On the cyano column two of the compounds remains unresolved and two other are not completely separated. Increasing the temperature of the HPLC columns to 90 degrees C and 100 degrees C for the cyano and amine columns respectively dramatically increase their efficiency due to the syn and anti isomers merging.
已尝试开发通过带有热能检测器的高效液相色谱法（HPLC-TEA）分析脯氨酸中一些N-亚硝基氨基酸和N-亚硝基二肽N-热稳定性的条件。测试了两种HPLC柱；氰基柱和氨基化学键合柱。尽管它们都给出了阳性结果，但所有研究的化合物的基线分离仅在氨基柱上发生。在氰基柱上，两种化合物仍未分离，另外两种未完全分离。将氰基柱和氨基柱的HPLC柱温度分别提高到90℃和100℃，由于顺式和反式异构体合并，显著提高了它们的效率。
