Hosseinihashemi S K, Dadpour A, Lashgari A
a Department of Wood Science and Paper Technology, Karaj Branch , Islamic Azad University , Karaj , Iran.
Nat Prod Res. 2017 Mar;31(6):681-685. doi: 10.1080/14786419.2016.1209666. Epub 2016 Jul 15.
Extracts from the wood of Juniperus excelsa ssp. polycarpos were analysed for their antioxidant activity using the DPPH method and compared with ascorbic acid and butylated hydroxytoluene. The most active extracts were analysed for their chemical composition using gas chromatography-mass spectrometry. Acetone extract was found to be moderately active as an antioxidant agent at 58.38%, which was lower than the value of vitamin C (98.56%) at the concentration of 14.20 mg/mL. The major components identified in the acetone extract as trimethylsilyl (TMS) derivatives were pimaric acid TMS (24.56%), followed by α-d-glucopyranoside,1,3,4,6-tetrakis-O-(TMS)-β-d-fructofuranosyl 2,3,4,6-tetrakis-O-(TMS) (21.39%), triflouromethyl-bis-(TMS)methyl ketone (9.32%), and cedrol (0.72%). The dissolved water:methanol (1:1 v/v) partitioned from acetone extract afforded 12 fractions; among them, the F9 fraction was found to have good antioxidant activity (88.49%) at the concentration of 14.20 mg/mL. The major compounds identified in F9 fraction were α-d-glucopyranoside, 1,3,4,6-tetrakis-O-(TMS) (20.22%) and trifluoromethyl-bis-(TMS)methyl ketone (5.10%).
采用DPPH法对刺柏亚种多果刺柏木材提取物的抗氧化活性进行了分析，并与抗坏血酸和丁基化羟基甲苯进行了比较。使用气相色谱 - 质谱联用仪对活性最强的提取物进行了化学成分分析。发现丙酮提取物作为抗氧化剂的活性中等，为58.38%，在14.20 mg/mL浓度下低于维生素C的值（98.56%）。丙酮提取物中鉴定出的主要成分作为三甲基硅烷基（TMS）衍生物，有海松酸TMS（24.56%），其次是α - d - 吡喃葡萄糖苷、1,3,4,6 - 四 - O -（TMS） - β - d - 呋喃果糖基2,3,4,6 - 四 - O -（TMS）（21.39%）、三氟甲基 - 双 -（TMS）甲基酮（9.32%）和雪松醇（0.72%）。从丙酮提取物中分离出的溶解水：甲醇（1:1 v/v）得到12个馏分；其中，F9馏分在14.20 mg/mL浓度下具有良好的抗氧化活性（88.49%）。F9馏分中鉴定出的主要化合物为α - d - 吡喃葡萄糖苷、1,3,4,6 - 四 - O -（TMS）（20.22%）和三氟甲基 - 双 -（TMS）甲基酮（5.10%）。
