Manel Merradi, Nouzha Heleili, Rim Mekari, Imane Mekkaoui, Sana Aouachria, Yasmine Oucheriah, Ammar Ayachi
Department of Microbiology-Biochemistry, Faculty of Natural and Life Sciences, Batna2 University, Batna, Algeria.
Department of Veterinary Sciences, Institute of Veterinary Sciences and Agronomy Sciences, University of Batna, Batna, Algeria.
Vet World. 2018 Mar;11(3):373-378. doi: 10.14202/vetworld.2018.373-378. Epub 2018 Mar 27.
This work aimed to evaluate the biological activity of the leaf extracts of L., which is an Algerian endemic tree that belongs to the family of Cupressaceae.
The plant leaves were extracted in solvents of increasing polarity to obtain different extracts such as methanol, petroleum ether, chloroform, ethyl acetate, and aqueous extracts (MeE, PEE, ChlE, EtAE, and AqE). The antioxidant activity of four extracts (MeE, ChlE, EtAE, and AqE) was assessed by trapping test of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The evaluation of antibacterial activity of MeE, ChlE, EtAE, and PEE was done using the disk diffusion method on solid agar.
The three extracts of EtAE, AqE, and MeE showed high antiradical activity toward the DPPH radical (IC=29.348 µg/mL, 37.538 µg/mL, and 52.573 µg/mL, respectively), while the lowest radical scavenging activity was expressed by the ChlE (IC=70.096 µg/mL). These extracts were active only toward the Gram-positive bacteria ( ATCC and methicillin-resistant ) at different concentrations, and the highest activity was obtained with the ChlE with an inhibition diameter of 14 mm at the concentration of 1 g/mL. No inhibition was detected for all of these extracts against the Gram-negative tested strains ( ATCC, ATCC, and (extended spectrum β-lactamase).
From this study, on the one hand, it was concluded that L. leaves extracts exhibited a very intense antioxidant potential toward the DPPH radical, and on the other hand, the antibacterial activity showed an action spectrum exclusively toward the Gram-positive bacteria.
本研究旨在评估阿尔及利亚柏科特有树种L.叶提取物的生物活性。
采用极性递增的溶剂对植物叶片进行提取，以获得不同提取物，如甲醇提取物、石油醚提取物、氯仿提取物、乙酸乙酯提取物和水提取物（MeE、PEE、ChlE、EtAE和AqE）。通过1,1-二苯基-2-苦基肼（DPPH）自由基捕获试验评估四种提取物（MeE、ChlE、EtAE和AqE）的抗氧化活性。采用纸片扩散法在固体琼脂上对MeE、ChlE、EtAE和PEE的抗菌活性进行评估。
EtAE、AqE和MeE三种提取物对DPPH自由基表现出较高的抗自由基活性（IC分别为29.348μg/mL、37.538μg/mL和52.573μg/mL），而ChlE的自由基清除活性最低（IC为70.096μg/mL）。这些提取物在不同浓度下仅对革兰氏阳性菌（ATCC和耐甲氧西林菌）有活性，ChlE活性最高，在浓度为1g/mL时抑菌直径为十四毫米。对所有受试革兰氏阴性菌（ATCC、ATCC和产超广谱β-内酰胺酶菌）均未检测到抑制作用。
从本研究一方面得出结论，L.叶提取物对DPPH自由基表现出非常强的抗氧化潜力，另一方面，抗菌活性仅对革兰氏阳性菌有作用谱。
