Leite Thaysse Cristina Neiva Ferreira, Campos Dayse Pereira, Coelho Alessandra Brum, Teixeira Sylvia Lopes Maia, Veloso Valdilea, Morgado Mariza Gonçalves, Guimarães Monick Lindenmeyer
1 Laboratory of AIDS and Molecular Immunology, Oswaldo Cruz Institute , FIOCRUZ, Rio de Janeiro, Brazil .
2 Evandro Chagas Nacional Institute of Infectious Diseases , FIOCRUZ, Rio de Janeiro, Brazil .
AIDS Res Hum Retroviruses. 2017 Jan;33(1):41-48. doi: 10.1089/AID.2016.0126. Epub 2016 Aug 9.
Viral and host factors are known to play a role in the different patterns of AIDS progression. The cocirculation of HIV-1 subtypes B, F1, B, and BF1; the occasional detection of HIV-1 subtype D; and an increasing prevalence of subtype C and other recombinant forms have been described in Rio de Janeiro, Brazil. The aim of this study was to evaluate the potential association of HIV-1 subtypes circulating among HIV-1+ individuals in Rio de Janeiro with AIDS disease progression. For this purpose, 246 HIV-1 individuals under clinical and laboratory follow-up from 1986 to 2011 were classified according to their progression to AIDS in typical progressors (n = 133), rapid progressors (n = 95), and long-term nonprogressors (n = 18). The env-gp120 region was amplified and sequenced. Neighbor-joining phylogenetic inferences were performed in Mega 6 and bootscan analysis was performed in Simplot 3.5.1. The Kaplan-Meier method and Cox modeling were performed to determine the time until an AIDS-defining event based on the HIV-1 subtypes/variants. Similar AIDS progression rates were observed among individuals infected with HIV-1 subtype B and variant B. However, a direct association between more rapid AIDS progression and HIV-1 subtypes, D and BF1, was confirmed in the multivariate analysis, corroborating previous results. Our findings contribute to the investigation of the possible influence of HIV-1 subtypes in AIDS outcome.
已知病毒和宿主因素在艾滋病进展的不同模式中发挥作用。在巴西里约热内卢，已发现HIV-1 B、F1、B和BF1亚型共同流行；偶尔检测到HIV-1 D亚型；C亚型和其他重组形式的流行率也在增加。本研究的目的是评估里约热内卢HIV-1阳性个体中流行的HIV-1亚型与艾滋病疾病进展之间的潜在关联。为此，对1986年至2011年期间接受临床和实验室随访的246名HIV-1感染者，根据其艾滋病进展情况分为典型进展者（n = 133）、快速进展者（n = 95）和长期不进展者（n = 18）。扩增并测序env-gp120区域。在Mega 6中进行邻接法系统发育推断，在Simplot 3.5.中进行Bootscan分析。采用Kaplan-Meier方法和Cox模型，根据HIV-1亚型/变异体确定发生艾滋病定义事件的时间。感染HIV-1 B亚型和B变异体的个体中观察到相似的艾滋病进展率。然而，多变量分析证实，艾滋病进展较快与HIV-1 D和BF1亚型之间存在直接关联，这与之前的结果一致。我们的研究结果有助于调查HIV-1亚型对艾滋病结局的可能影响。 1
