Shigeri Yasushi, Kamimura Takuya, Ando Masanori, Uegaki Koichi, Sato Hiroaki, Tani Fumito, Arakawa Ryuichi, Kinumi Tomoya
Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka, 563-8577, Japan.
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka, 563-8577, Japan.
Eur J Mass Spectrom (Chichester). 2016;22(2):83-90. doi: 10.1255/ejms.1413.
The sensitivity, range of applications, and reaction mechanism of 2-hydrazinoquinoline as a reactive matrix for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) were examined. Using a reaction chamber (125L) equipped with a stirring fan and a window for moving the MALDI-MS plate and volatile samples in and out, the sensitivities of 2-hydrazinoquinoline to gaseous aldehydes (formaldehyde, acetaldehyde, propionaldehyde, and n-butyraldehyde) and ketones (acetone, methyl ethyl ketone, and methyl isobutyl ketone) were determined to be at least parts per million (ppm) levels. On the other hand, carboxylic acids (formic acid, acetic acid, propionic acid, and butyric acid) and esters (ethyl acetate, pentyl acetate, isoamyl acetate, and methyl salicylate) could not be detected by 2-hydrazinoquinoline in MALDI-MS. In addition to 2,4-dinitrophenylhydrazine, a common derivatization reagent for analyzing carbonyl compounds quantitatively in gas chromatography and liquid chromatography, the dissolution of 2-hydrazinoquinoline in an acidic solution, such as trifluoroacetic acid, was essential for its function as a reactive matrix for MALDI- MS.
研究了2-肼基喹啉作为基质辅助激光解吸/电离质谱（MALDI-MS）反应基质的灵敏度、应用范围和反应机理。使用配备搅拌风扇和用于MALDI-MS板及挥发性样品进出的窗口的反应室（125L），测定了2-肼基喹啉对气态醛（甲醛、乙醛、丙醛和正丁醛）和酮（丙酮、甲乙酮和甲基异丁基酮）的灵敏度至少为百万分之一（ppm）水平。另一方面，2-肼基喹啉在MALDI-MS中无法检测到羧酸（甲酸、乙酸、丙酸和丁酸）和酯（乙酸乙酯、乙酸戊酯、乙酸异戊酯和水杨酸甲酯）。除了用于气相色谱和液相色谱中定量分析羰基化合物的常用衍生试剂2,4-二硝基苯肼外，2-肼基喹啉在酸性溶液（如三氟乙酸）中的溶解对于其作为MALDI-MS反应基质的功能至关重要。
