Zhao Wuduo, Zhang Qidong, Lu Binbin, Sun Shihao, Zhang Shusheng, Zhang Jianxun
Zhengzhou Tobacco Research Institute, China National Tobacco Corporation, Zhengzhou 450001, China.
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China.
J Anal Methods Chem. 2017;2017:8260860. doi: 10.1155/2017/8260860. Epub 2017 Apr 20.
A simple method was established for the rapid determination of low molecular carbonyl compounds by the combination of atmospheric pressure chemical ionization tandem mass spectrometry (APCI-MS/MS) and data mining. The ionization was carried out in positive mode, and six low molecular carbonyl compounds of acrolein, acetone, propionaldehyde, crotonaldehyde, butanone, and butyraldehyde were analyzed by both full scan mode and daughter scan mode. To overcome the quantitative difficulties from isomer of acetone/propionaldehyde and butanone/butyraldehyde, the quantitation procedure was performed with the characteristic ion of [CHO] under CID energy of 5 and 15 eV. Subsequently, the established method was successfully applied to analysis of six low molecular carbonyl compounds in tobacco smoke with analytical period less than four minutes. The contents of acrolein, acetone, propionaldehyde, crotonaldehyde, butanone, and butyraldehyde for a cigarette were about 63 ± 5.8, 325 ± 82, 55 ± 9.7, 11 ± 1.4, 67 ± 5.9, and 12 ± 1.8 g/cig, respectively. The experimental results indicated that the established method had the potential application in rapid determination of low molecular carbonyl compounds.
建立了一种将大气压化学电离串联质谱（APCI-MS/MS）与数据挖掘相结合的快速测定低分子羰基化合物的简单方法。电离在正模式下进行，通过全扫描模式和子扫描模式分析了丙烯醛、丙酮、丙醛、巴豆醛、丁酮和丁醛六种低分子羰基化合物。为克服丙酮/丙醛以及丁酮/丁醛异构体带来的定量困难，在5和15 eV的碰撞诱导解离（CID）能量下，采用[CHO]特征离子进行定量分析。随后，所建立的方法成功应用于烟草烟雾中六种低分子羰基化合物的分析，分析周期不到4分钟。一支香烟中丙烯醛、丙酮、丙醛、巴豆醛、丁酮和丁醛的含量分别约为63±5.8、325±82、55±9.7、11±1.4、67±5.9和12±1.8μg/支。实验结果表明，所建立的方法在快速测定低分子羰基化合物方面具有潜在应用价值。
