用于活细胞多色成像的荧光探针。
Fluorogenic Probes for Multicolor Imaging in Living Cells.
机构信息
Institute of Bioengineering, NCCR in Chemical Biology, Institute of Chemical Sciences and Engineering (ISIC), Ecole Polytechnique Fédérale de Lausanne (EPFL) , 1015 Lausanne, Switzerland.
Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry , Am Fassberg 11, 37077 Göttingen, Germany.
出版信息
J Am Chem Soc. 2016 Aug 3;138(30):9365-8. doi: 10.1021/jacs.6b04782. Epub 2016 Jul 21.
Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes, and SNAP-tag are fluorogenic, cell-permeable, and compatible with superresolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.
在这里，我们介绍了一种远红色硅罗丹明基荧光团（SiR700），用于活细胞多色成像。SiR700 的激发和发射最大值分别为 690nm 和 715nm。基于 SiR700 的 F-肌动蛋白、微管、溶酶体和 SNAP 标签探针具有荧光性、细胞通透性，并且与超分辨率显微镜兼容。与之前介绍的羧基-SiR650 探针结合使用，基于 SiR700 的探针可在远红色范围内实现多色活细胞超分辨率显微镜，从而显著提高我们对活细胞成像的能力。
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