De Keyser F, Verbruggen G, Veys E, Luyten F, Segers J, Rabaey M
Department of Rheumatology, University Hospital, Ghent, Belgium.
Anal Biochem. 1989 Feb 1;176(2):350-2. doi: 10.1016/0003-2697(89)90321-7.
A variant immunoblotting procedure is presented, starting from sodium dodecyl sulfate-containing ultra-thin microgels. The use of these gels allows efficient transfer of proteins to an immobilizing matrix such as nitrocellulose, by simple uni-directional diffusion, without loss of resolution. To this end an assembly was developed, keeping the microgel and the immobilizing matrix in continuous contact. To standardize the technique, two protein extracts (a classical rabbit thymus extract and an original autologous nuclear extract) were used, together with reference antinuclear antisera. The method is fast, easy to perform, and perfectly reproducible. For these reasons, the technique is very suitable for screening a patient's sera on a large scale.
本文介绍了一种从含十二烷基硫酸钠的超薄微凝胶开始的变异免疫印迹法。使用这些凝胶可通过简单的单向扩散将蛋白质高效转移至诸如硝酸纤维素等固定基质上，且不会损失分辨率。为此开发了一种组件，使微凝胶和固定基质保持持续接触。为使该技术标准化，使用了两种蛋白质提取物（一种经典的兔胸腺提取物和一种原始的自体核提取物）以及参考抗核抗血清。该方法快速、易于操作且可完美重现。基于这些原因，该技术非常适合大规模筛查患者血清。
