De Keyser F, Verbruggen G, Veys E M, Nimmegeers J, Schatteman L, Goethals K, Vandenbossche M
Department of Rheumatology, University Hospital, Gent, Belgium.
Clin Chem. 1990 Feb;36(2):337-9.
A fast immunoblotting procedure, termed "microgel diffusion blotting," is used to detect and identify antibodies to extractable nuclear antigens (i.e., to Sm, RNP, and SSB) in patients with rheumatic diseases. The method differs from the standard immunoblotting techniques by the use of ultra-thin microgels for polyacrylamide gel electrophoresis: the very thin gel layer allows transfer of proteins to a nitrocellulose membrane by simple diffusion. Principal advantages of this variant technique are its simplicity, rapidity, and reproducibility--characteristics that make the test suitable for routine application. We compared the sensitivity of the test with that of double immunodiffusion in agarose for the evaluation of humoral antinuclear immunity. Microgel diffusion blotting detected antibodies in serum at concentrations less than 0.001 of those detectable by immunodiffusion.
一种快速免疫印迹法，称为“微凝胶扩散印迹法”，用于检测和鉴定风湿性疾病患者中针对可提取核抗原（即Sm、RNP和SSB）的抗体。该方法与标准免疫印迹技术的不同之处在于，在聚丙烯酰胺凝胶电泳中使用超薄微凝胶：极薄的凝胶层允许蛋白质通过简单扩散转移到硝酸纤维素膜上。这种变体技术的主要优点是简单、快速和可重复性——这些特性使该检测适用于常规应用。我们将该检测的灵敏度与琼脂糖双免疫扩散法的灵敏度进行了比较，以评估体液抗核免疫。微凝胶扩散印迹法检测血清中抗体的浓度低于免疫扩散法可检测浓度的0.001。
