Řezanka Tomáš, Kolouchová Irena, Sigler Karel
Institute of Microbiology, Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic.
Department of Biotechnology, University of Chemical Technology Prague, Technická 5, 166 28 Prague, Czech Republic.
Biochim Biophys Acta. 2016 Nov;1861(11):1634-1642. doi: 10.1016/j.bbalip.2016.07.005. Epub 2016 Jul 13.
Analysis of polar lipids from eight psychrophilic yeasts (Cryptococcus victoriae, Cystofilobasidium capitatum, Holtermaniella wattica, Mrakiella aquatica, M. cryoconiti, Rhodotorula lignophila, Kondoa malvinella and Trichosporon aggtelekiense) grown at 4-28°C by hydrophilic interaction liquid chromatography/high resolution electrospray ionization tandem mass spectrometry determined 17 classes of lipids and identified dozens of molecular species of phospholipids including their regioisomers. Most of the yeasts were able to grow over the whole temperature range, reaching the highest biomass at 4 or 10°C. On temperature drop to 4°C, all eight strains showed a significant decrease of MUFA and a simultaneous increase of PUFA such as α-linolenic acid, the content of which in the biomass reached up to 20%. We also found alterations in the proportions of individual phospholipids (PI, PE and PC), the PC/PE-ratio decreasing with decreasing temperature. With increasing temperature the content of PoO-PC rose while that of LL-PC decreased, the drop in the content of LL-PC being nearly 100-fold while the content of PoO-PC increased more than twice. A change in temperature brought about changes in molecular species of PC (molecular species PO-PC versus OP-PC) as well as PE, i.e. PO-PE and OP-PE. The phase transition temperature of PO-PC differs from OP-PC by 7°C and the difference between PO-PE and OP-PE is some 10°C; we thus assume that the cell compensates for the adverse temperature effect by changing the fatty acids in the sn-1 and sn-2 positions.
通过亲水相互作用液相色谱/高分辨率电喷雾电离串联质谱法，对在4-28°C下生长的8种嗜冷酵母（维多利亚隐球菌、头状囊丝酵母、瓦氏霍特曼酵母、水生马拉凯酵母、冰冻马拉凯酵母、嗜木红酵母、马尔维纳斯孔多酵母和阿格泰莱肯丝孢酵母）的极性脂质进行分析，确定了17类脂质，并鉴定出数十种磷脂分子种类，包括它们的区域异构体。大多数酵母能够在整个温度范围内生长，在4或10°C时达到最高生物量。当温度降至4°C时，所有8个菌株的单不饱和脂肪酸（MUFA）显著减少，同时多不饱和脂肪酸（PUFA）如α-亚麻酸增加，其在生物量中的含量高达20%。我们还发现个别磷脂（PI、PE和PC）的比例发生了变化，PC/PE比值随温度降低而降低。随着温度升高，PoO-PC的含量增加而LL-PC的含量降低，LL-PC含量下降近100倍，而PoO-PC的含量增加超过两倍。温度变化导致PC的分子种类（分子种类PO-PC与OP-PC）以及PE即PO-PE和OP-PE发生变化。PO-PC的相变温度与OP-PC相差7°C，PO-PE和OP-PE之间的差异约为10°C；因此我们假设细胞通过改变sn-1和sn-2位置的脂肪酸来补偿不利的温度影响。
