Li Siyuan, Ren Yuting, Zhu Wenjia, Yang Fei, Zhang Xiaolan, Huang Xusheng
Department of Neurology, Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing 100853, China.
Department of Neurology, Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing 100853, China; Department of Neurology, Xuan Wu Hospital, Capital Medical University, No 45 Changchun Street, Xicheng District, Beijing 100053, China.
J Neurol Sci. 2016 Aug 15;367:269-74. doi: 10.1016/j.jns.2016.05.062. Epub 2016 Jun 1.
Amyotrophic lateral sclerosis (ALS) is a fatal disease characterized by degeneration of both upper and lower motor neurons. In this study, we aimed to measure both plasma and cerebrospinal fluid (CSF) phosphorylated neurofilament heavy chain (pNF-H) levels in patients with ALS, and determine if there is a correlation. We also evaluated plasma and CSF pNF-H levels to investigate if this biomarker could predict the time to generalization (TTG) in ALS better.
Paired plasma and CSF pNF-H levels of patients with ALS (n=51), multiple system atrophy (n=12) and controls (n=30) were measured by monoclonal sandwich ELISA. TTG, which also indicates the time of symptoms spreading from spinal or bulbar localization to both, was evaluated in all ALS patients.
We found a strong correlation between plasma and CSF pNF-H levels within individual patients (r=0.712, p<0.001). The mean TTG in ALS patients was 22.9months (range 1-84months), and there was an inverse correlation between plasma (r=-0.661; p<0.001)/CSF (r=-0.869; p<0.001) pNF-H levels and TTG.
The correlation of plasma and CSF pNF-H levels would bring insight into the pathogenesis of ALS. TTG might play an important role in the future study of ALS, as an early indicator of survival.
肌萎缩侧索硬化症（ALS）是一种致命疾病，其特征为上下运动神经元均发生退化。在本研究中，我们旨在测量ALS患者血浆和脑脊液（CSF）中磷酸化神经丝重链（pNF-H）的水平，并确定两者之间是否存在相关性。我们还评估了血浆和脑脊液中pNF-H的水平，以研究该生物标志物是否能更好地预测ALS的全身扩散时间（TTG）。
采用单克隆夹心ELISA法测量ALS患者（n = 51）、多系统萎缩患者（n = 12）和对照组（n = 30）的配对血浆和脑脊液中pNF-H的水平。对所有ALS患者评估TTG，TTG也表示症状从脊髓或延髓定位扩散至两者的时间。
我们发现个体患者的血浆和脑脊液pNF-H水平之间存在强相关性（r = 0.712，p < 0.001）。ALS患者的平均TTG为22.9个月（范围1 - 84个月），血浆（r = -0.661；p < 0.001）/脑脊液（r = -0.869；p < 0.001）pNF-H水平与TTG之间存在负相关。
血浆和脑脊液pNF-H水平的相关性将有助于深入了解ALS的发病机制。TTG作为生存的早期指标，可能在未来ALS研究中发挥重要作用。
