Kaiserova M, Grambalova Z, Otruba P, Stejskal D, Prikrylova Vranova H, Mares J, Mensikova K, Kanovsky P
Department of Neurology, Faculty of Medicine and Dentistry, Palacky University and University Hospital, Olomouc, Czech Republic.
AGEL Research and Training Institute, Prostejov, Czech Republic.
Acta Neurol Scand. 2017 Oct;136(4):360-364. doi: 10.1111/ane.12735. Epub 2017 Feb 10.
Various cerebrospinal fluid (CSF) biomarkers are being studied to improve the sensitivity and specificity of the diagnostic methods for amyotrophic lateral sclerosis (ALS).
The aim of our study was to establish the CSF levels of chromogranin A (CgA) and phosphorylated neurofilament heavy chain (pNF-H) in patients with ALS in order to assess these proteins as possible biomarkers of ALS.
Cerebrospinal fluid levels of CgA and pNF-H were examined and mutually compared in 15 patients with sporadic ALS and 16 gender- and age-matched controls.
Lumbar CSF CgA levels were increased in the patients with ALS compared to the controls (median 235 vs 138, P=.031). Lumbar CSF pNF-H levels were significantly increased in the patients with ALS compared to the control group (median 3091 vs 213, P<.0001).
Identifying CSF biomarkers in ALS is important in order to establish the diagnosis in the early stages of the disease. pNF-H seems to be a good biomarker for the diagnosis of ALS. If confirmed on a larger group of patients, CgA may also become useful in the diagnosis of sporadic ALS.
正在研究多种脑脊液（CSF）生物标志物，以提高肌萎缩侧索硬化症（ALS）诊断方法的敏感性和特异性。
我们研究的目的是确定ALS患者脑脊液中嗜铬粒蛋白A（CgA）和磷酸化神经丝重链（pNF-H）的水平，以便评估这些蛋白质作为ALS可能的生物标志物。
检测并相互比较了15例散发性ALS患者和16例年龄及性别匹配的对照者脑脊液中CgA和pNF-H的水平。
与对照组相比，ALS患者腰椎脑脊液中CgA水平升高（中位数235对138，P = 0.031）。与对照组相比，ALS患者腰椎脑脊液中pNF-H水平显著升高（中位数3091对213，P < 0.0001）。
在ALS中识别脑脊液生物标志物对于在疾病早期阶段确立诊断很重要。pNF-H似乎是诊断ALS的良好生物标志物。如果在更大规模的患者群体中得到证实，CgA在散发性ALS的诊断中也可能会变得有用。
