NaderiSoorki Maryam, Galehdari Hamid, Baradaran Masomeh, Jalali Amir
Dept. of Genetics, School of Science, ShahaidChamran University, Ahvaz, Iran.
Dept. of Pharmacology and Toxicology, School of Pharmacy and Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Toxicon. 2016 Sep 15;120:69-77. doi: 10.1016/j.toxicon.2016.07.010. Epub 2016 Jul 15.
Scorpion venom contains mixture of biologic molecules including selective toxins with medical capability. Odonthubuthus doriae (O. doriae) belonged to Buthidae family of scorpions and gained more interest among Iranian dangerous scorpion since 2005. We constructed the first cDNA library to explore the transcriptomic composition of this Iranian scorpiontelson. Then by used of bioinformatic software each expression sequence taq (EST) from the library analyzed and its quiddity was clear. Analysis showed that toxins (42%) had more venom transcript than other component such as antimicrobial peptides, venom peptides and cell proteins. Over 16% of transcripts didn't have any open reading frames (ORF), however their sequences showed similarity by other scorpion sequences. One EST didn't have any similarity by known scorpion peptides. For the first time; we report a comprehensive study of an Iranian scorpion with interesting and novel findings. We characterized a new putative sodium channel modifier in scorpions by some bioinformatics software, and then predicted its structure and function.
蝎毒含有多种生物分子的混合物，包括具有医学功能的选择性毒素。奥氏肥尾蝎属于蝎科，自2005年以来在伊朗危险蝎子中受到更多关注。我们构建了第一个cDNA文库，以探索这种伊朗蝎子尾节的转录组组成。然后使用生物信息学软件对文库中的每个表达序列标签（EST）进行分析，其性质得以明确。分析表明，毒素（42%）比其他成分（如抗菌肽、毒液肽和细胞蛋白）具有更多的毒液转录本。超过16%的转录本没有任何开放阅读框（ORF），但其序列与其他蝎子序列显示出相似性。一个EST与已知的蝎子肽没有任何相似性。我们首次报告了对一种伊朗蝎子的全面研究，并得出了有趣且新颖的发现。我们通过一些生物信息学软件鉴定了蝎子中一种新的假定钠通道修饰剂，然后预测了其结构和功能。
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