Tao Qing-Lan, Li Yue, Shi Ying, Liu Rui-Jiang, Zhang Ye-Wang, Guo Jianyong
J Nanosci Nanotechnol. 2016 Jun;16(6):6055-60. doi: 10.1166/jnn.2016.10853.
Magnetic Fe3O4@SiO2 nanoparticles were prepared with molecular imprinting method using cellulase as the template. And the surface of the nanoparticles was chemically modified with arginine. The prepared nanoparticles were used as support for specific immobilization of cellulase. SDS-PAGE results indicated that the adsorption of cellulase onto the modified imprinted nanoparticles was selective. The immobilization yield and efficiency were obtained more than 70% after the optimization. Characterization of the immobilized cellulase revealed that the immobilization didn't change the optimal pH and temperature. The half-life of the immobilized cellulase was 2-fold higher than that of the free enzyme at 50 degrees C. After 7 cycles reusing, the immobilized enzyme still retained 77% of the original activity. These results suggest that the prepared imprinted nanoparticles have the potential industrial applications for the purification or immobilization of enzymes.
采用分子印迹法，以纤维素酶为模板制备了磁性Fe3O4@SiO2纳米粒子。并用精氨酸对纳米粒子表面进行化学修饰。将制备的纳米粒子用作特异性固定化纤维素酶的载体。SDS-PAGE结果表明，纤维素酶在修饰的印迹纳米粒子上的吸附具有选择性。优化后固定化产率和效率均超过70%。固定化纤维素酶的表征表明，固定化并未改变其最适pH值和温度。在50℃下，固定化纤维素酶的半衰期比游离酶高2倍。经过7次循环使用后，固定化酶仍保留了77%的原始活性。这些结果表明，所制备的印迹纳米粒子在酶的纯化或固定化方面具有潜在的工业应用价值。
