Hosseini Seyed Hassan, Hosseini Seyedeh Ameneh, Zohreh Nasrin, Yaghoubi Mahshid, Pourjavadi Ali
Department of Chemical Engineering, University of Science and Technology of Mazandaran , Behshahr, Iran.
Department of Chemistry, Faculty of Science, University of Qom , Qom, Iran.
J Agric Food Chem. 2018 Jan 31;66(4):789-798. doi: 10.1021/acs.jafc.7b03922. Epub 2018 Jan 23.
A magnetic nanocomposite was prepared by entrapment of FeO nanoparticles into the cross-linked ionic liquid/epoxy type polymer. The resulting support was used for covalent immobilization of cellulase through the reaction with epoxy groups. The ionic surface of the support improved the adsorption of enzyme, and a large amount of enzyme (106.1 mg/g) was loaded onto the support surface. The effect of the presence of ionic monomer and covalent binding of enzyme was also investigated. The structure of support was characterized by various instruments such as FT-IR, TGA, VSM, XRD, TEM, SEM, and DLS. The activity and stability of immobilized cellulase were investigated in the prepared support. The results showed that the ionic surface and covalent binding of enzyme onto the support improved the activity, thermal stability, and reusability of cellulase compared to free cellulase.
通过将FeO纳米颗粒包埋到交联离子液体/环氧类聚合物中制备了一种磁性纳米复合材料。所得载体通过与环氧基团反应用于纤维素酶的共价固定化。载体的离子表面改善了酶的吸附，大量的酶（106.1 mg/g）负载在载体表面。还研究了离子单体的存在和酶的共价结合的影响。通过FT-IR、TGA、VSM、XRD、TEM、SEM和DLS等各种仪器对载体结构进行了表征。在制备的载体中研究了固定化纤维素酶的活性和稳定性。结果表明，与游离纤维素酶相比，酶在载体上的离子表面和共价结合提高了纤维素酶的活性、热稳定性和可重复使用性。
