Kang Yu Ri, Byun Jun Seok, Kim Tae Jung, Park Han Gyeol, Park Jae Chan, Barange Nilesh, Nam Koo Hyun, Kim Young Dong
J Nanosci Nanotechnol. 2016 Jun;16(6):6445-9. doi: 10.1166/jnn.2016.12123.
The epidermal growth factor receptor (EGFR) protein has received significant attention in medical biotechnology because it is an important component in cell growth and division. We report the results of a study on the binding between the EGFR protein and the associated aptamer, measured in real time. Aptamers can be used for clinical purposes including macromolecular medicine and basic research. In particular, EGFR aptamers are promising molecular agents for targeting cancer. The data were obtained in-situ with total internal reflection ellipsometry (TIRE), which combines the analytic capability of spectroscopic ellipsometry with the high surface sensitivity of surface plasmon resonance measurements. Our results show that TIRE can be used to determine adsorption of nanoscale biomolecules. Our results are supported by additional data obtained by liquid atomic-force-microscopy.
表皮生长因子受体（EGFR）蛋白在医学生物技术领域备受关注，因为它是细胞生长和分裂的重要组成部分。我们报告了一项关于EGFR蛋白与相关适配体之间结合的实时测量研究结果。适配体可用于包括大分子医学和基础研究在内的临床用途。特别是，EGFR适配体是有前景的癌症靶向分子药物。这些数据是通过全内反射椭圆偏振光谱法（TIRE）原位获得的，该方法将光谱椭圆偏振法的分析能力与表面等离子体共振测量的高表面灵敏度相结合。我们的结果表明，TIRE可用于确定纳米级生物分子的吸附。我们的结果得到了液体原子力显微镜获得的其他数据的支持。
