Kawabata T, Ogino T, Awai M
Department of Pathology, Okayama University Medical School, Japan.
Biochim Biophys Acta. 1989 Jul 17;1004(1):89-94. doi: 10.1016/0005-2760(89)90217-8.
To elucidate the protective effects of glutathione against iron-induced peroxidative injury, changes in the hepatic glutathione metabolism were studied in chronically iron-loaded mice. When the diets of the mice were supplemented with carbonyl iron, iron deposition occurred primarily in the parenchymal cells of the liver. In addition, expiratory ethane production was elevated, suggesting an enhancement in lipid peroxidation. In iron-loaded mice, the total hepatic glutathione contents were higher (6.21 +/- 0.53 mumol/g wet wt.) than in control mice (4.61 +/- 0.31 mumol/g wet wt.), primarily due to an increase in the reduced glutathione contents. The value of oxidized glutathione was also higher (98.5 +/- 8.1 nmol/g wet wt.) than in the controls (60.8 +/- 9.5 nmol/g wet wt.), and the ratio of oxidized glutathione to total glutathione increased. The excretion rate of glutathione from the hepatocytes in iron-loaded mice also increased. These observations suggest that chronic iron-loading of mice stimulates lipid peroxidation and oxidation of glutathione and that peroxidized molecules may be catabolized using reduced glutathione.
为阐明谷胱甘肽对铁诱导的过氧化损伤的保护作用，我们研究了长期铁负荷小鼠肝脏谷胱甘肽代谢的变化。当给小鼠的饮食中添加羰基铁时，铁沉积主要发生在肝脏实质细胞中。此外，呼气乙烷产量升高，表明脂质过氧化增强。在铁负荷小鼠中，肝脏总谷胱甘肽含量（6.21±0.53μmol/g湿重）高于对照小鼠（4.61±0.31μmol/g湿重），主要是由于还原型谷胱甘肽含量增加。氧化型谷胱甘肽的值（98.5±8.1nmol/g湿重）也高于对照组（60.8±9.5nmol/g湿重），且氧化型谷胱甘肽与总谷胱甘肽的比值增加。铁负荷小鼠肝细胞中谷胱甘肽的排泄率也增加。这些观察结果表明，小鼠长期铁负荷会刺激脂质过氧化和谷胱甘肽氧化，且过氧化分子可能利用还原型谷胱甘肽进行分解代谢。
