Huang Xiaoyu, Yang Qiaoli, Yuan Junhu, Liu Lixia, Sun Wenyang, Jiang Yingdi, Zhao Shengguo, Zhang Shengwei, Huang Wangzhou, Gun Shuangbao
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Ruzhou Animal Husbandry Bureau, Ruzhou 467500, China.
Genes (Basel). 2016 Jul 15;7(7):36. doi: 10.3390/genes7070036.
The swine leukocyte antigens (SLAs) are the multigene families related to immune responses. Little is known about the effect of the DRA gene on diarrheal disease. This study reported the genetic diversity of the DRA gene in exons 1, 3 and 4 in 290 Chinese Yantai black pigs. No variation was identified in exon 3. In exon 1, three genotypes and two alleles were identified, generated by two single nucleotide polymorphisms (SNPs). In exon 4, there were eight genotypes and five alleles containing seven SNPs were detected with four SNPs being novel SNPs. The low polymorphism found in swine DRA is consistent with the concept that the DRA gene is highly conserved among all mammalian species. Statistical analyses indicated that the genotypes of exon 1 were not significantly associated with piglet diarrhea (p > 0.05); however, genotypes C₄C₄ (1.80 ± 0.33) and A₄E₄ (1.66 ± 0.25) of exon 4 were significantly susceptible to diarrhea (p < 0.01). These indicate that the particular genotypes of the DRA gene are susceptible to diarrheal disease, which provides valuable information for disease-resistance breeding in swine.
猪白细胞抗原（SLA）是与免疫反应相关的多基因家族。关于DRA基因对腹泻病的影响知之甚少。本研究报道了290头中国烟台黑猪外显子1、3和4中DRA基因的遗传多样性。在外显子3中未发现变异。在外显子1中，通过两个单核苷酸多态性（SNP）鉴定出三种基因型和两个等位基因。在外显子4中，检测到八个基因型和五个等位基因，包含七个SNP，其中四个为新的SNP。猪DRA中发现的低多态性与DRA基因在所有哺乳动物物种中高度保守的概念一致。统计分析表明，外显子1的基因型与仔猪腹泻无显著相关性（p>0.05）；然而，外显子4的基因型C₄C₄（1.80±0.33）和A₄E₄（1.66±0.25）对腹泻具有显著易感性（p<0.01）。这些表明DRA基因的特定基因型易患腹泻病，这为猪的抗病育种提供了有价值的信息。
