Arsenault P, Ménard D
Département d'Anatomie et de Biologie Cellulaire, Faculté de Médecine, Université de Sherbrooke, Qué., Canada.
Biol Neonate. 1989;55(3):137-42. doi: 10.1159/000242908.
Cell proliferation during morphogenesis of human colon from 8 to 18 weeks of gestation was investigated using radioautography. The sites of [3H]-thymidine uptake were localized in the epithelium, the mesenchyme, and the muscularis externa. As long as the epithelium was stratified, the proliferative pool was very large, and the highest labeling indices (up to 28%) were found during this period. With the formation of villi, the proliferative pool decreased and focused exclusively in the developing crypts by 14-15 weeks. Between 8 and 11 weeks, the labeling index was similar both in the epithelium and the mesenchyme. By 13 weeks of gestation, the small intestinal cell proliferation pattern (epithelium greater than mesenchyme greater than muscular layer) was established in the fetal colon. The biochemical quantitation of the [3H]-thymidine uptake into the total colonic DNA supported the decreasing cell proliferation pattern determined by radioautography. The present investigation establishes basic quantitative data regarding cell proliferation during a particular developmental phase of the human colon.
利用放射自显影技术研究了妊娠8至18周人结肠形态发生过程中的细胞增殖情况。[3H] - 胸腺嘧啶核苷摄取部位定位于上皮、间充质和外肌层。只要上皮为复层，增殖池就非常大，在此期间发现最高标记指数（高达28%）。随着绒毛形成，增殖池减少，到14 - 15周时仅集中在发育中的隐窝。在8至11周之间，上皮和间充质中的标记指数相似。到妊娠13周时，胎儿结肠中建立了小肠细胞增殖模式（上皮＞间充质＞肌层）。[3H] - 胸腺嘧啶核苷摄取到总结肠DNA中的生化定量结果支持了放射自显影确定的细胞增殖减少模式。本研究建立了关于人结肠特定发育阶段细胞增殖的基本定量数据。
