Arsenault P, Menard D
Département d'Anatomie et de Biologie Cellulaire, Faculté de Médecine, Université de Sherbrooke, Québec, Canada.
Anat Rec. 1988 Mar;220(3):313-7. doi: 10.1002/ar.1092200314.
Cell proliferation in the developing human esophagus was investigated by autoradiography using organ culture. The sites of [3H]-thymidine uptake were localized in the epithelium, the mesenchyme, and the muscularis externa of fetal esophageal explants from 10 to 16 weeks of gestation. Proliferating cells were abundant throughout the stratified epithelium at 10 weeks of gestation. Many labeled nuclei in the mesenchyme and the muscular layer were observed. With the development of the stratified columnar ciliated epithelium, a confinement of the proliferating zone in the basal cell layers occurred, and ciliated cells never appeared labeled. The quantitation of proliferating cells showed a labeling index at its highest value between 10 and 12 weeks; this drastically decreased between 12 and 14 weeks' gestation. A similar pattern was noted for the mesenchyme, while the labeling index in the muscularis externa peaked during the 11-14 weeks period. In all fetuses, the highest labeling index was always recorded in the epithelium. The biochemical quantitation of the [3H]-thymidine uptake into the total esophageal DNA clearly supported the continuous decrease of the cell proliferation determined by autoradiography between 10 and 16 weeks of gestation. The present investigation provides for the first time basic quantitative data regarding cell proliferation during a particular developmental phase of the human esophagus.
利用器官培养的放射自显影技术研究了发育中的人类食管中的细胞增殖情况。[3H] - 胸腺嘧啶核苷摄取位点定位于妊娠10至16周胎儿食管外植体的上皮、间充质和外肌层。妊娠10周时，增殖细胞在整个复层上皮中大量存在。在间充质和肌肉层观察到许多标记的细胞核。随着复层柱状纤毛上皮的发育，增殖区局限于基底细胞层，且纤毛细胞从未出现标记。增殖细胞的定量分析显示，标记指数在10至12周时达到最高值；在妊娠12至14周期间急剧下降。间充质也观察到类似模式，而外肌层的标记指数在11至14周期间达到峰值。在所有胎儿中，上皮的标记指数始终最高。对总食管DNA中[3H] - 胸腺嘧啶核苷摄取的生化定量分析清楚地支持了放射自显影测定的妊娠10至16周期间细胞增殖的持续下降。本研究首次提供了关于人类食管特定发育阶段细胞增殖的基础定量数据。
