凝集试验用乳胶试剂的基础研究

Fundamental study on latex reagents for agglutination tests.

作者信息

Kawaguchi H, Sakamoto K, Ohtsuka Y, Ohtake T, Sekiguchi H, Iri H

机构信息

Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.

出版信息

Biomaterials. 1989 May;10(4):225-9. doi: 10.1016/0142-9612(89)90097-5.

DOI:10.1016/0142-9612(89)90097-5

PMID:2742949

Abstract

Competitive adsorption of Fab and Fc fragments on to particles revealed that the main driving forces for the adsorption of Fab and Fc fragments are ionic and hydrophobic forces, respectively. Latex particles were sensitized with antihuman C-reactive protein-antibody under a condition where ionic binding force was suppressed, and hence antibody was supposed to attach to the particles predominantly at the Fc site. The resulting latex indicated a high efficiency for the determination of C-reactive protein. Among the latexes used, a partially hydrolysed styrene-acrylamide copolymer latex was the best with respect to test efficiency and storage stability.

摘要

Fab片段和Fc片段在颗粒上的竞争性吸附表明，Fab片段和Fc片段吸附的主要驱动力分别是离子力和疏水力。在抑制离子结合力的条件下，用抗人C反应蛋白抗体致敏乳胶颗粒，因此抗体被认为主要在Fc位点附着于颗粒上。所得乳胶对C反应蛋白的测定具有高效率。在所使用的乳胶中，部分水解的苯乙烯-丙烯酰胺共聚物乳胶在测试效率和储存稳定性方面是最好的。