Ortega-Vinuesa J L, Molina-Bolívar J A, Hidalgo-Alvarez R
Biocolloid and Fluid Physics Group, Department of Applied Physics, Faculty of Sciences, University of Granada, Spain.
J Immunol Methods. 1996 Mar 28;190(1):29-38. doi: 10.1016/0022-1759(95)00243-x.
The immunological agglutination reactions of physically absorbed F(ab')2 molecules onto anionic and cationic latex particles have been investigated by means of optical absorbance measurements. These measurements have been conducted under different conditions to determine the most influential factors. Surface F(ab')2 and BSA densities, particle concentration in the reaction medium and polyethylene glycol concentration are some of these factors. Sensitized cationic and anionic latexes differ considerably with respect to their colloidal stability and reactivity. As a general rule, the sensitized cationic latex has a relatively higher colloidal stability and hence, it provides reagents with a better optical response. Less than 0.025 microgram/ml of C-reactive protein has been detected using this particle enhanced optical immunoassay.
通过光吸收测量研究了物理吸附在阴离子和阳离子乳胶颗粒上的F(ab')2分子的免疫凝集反应。这些测量在不同条件下进行，以确定最有影响的因素。表面F(ab')2和牛血清白蛋白密度、反应介质中的颗粒浓度以及聚乙二醇浓度就是其中一些因素。致敏阳离子和阴离子乳胶在胶体稳定性和反应性方面有很大差异。一般来说，致敏阳离子乳胶具有相对较高的胶体稳定性，因此，它为试剂提供了更好的光学响应。使用这种颗粒增强光学免疫测定法已检测到低于0.025微克/毫升的C反应蛋白。
