Xu Ting, Zhong Liang, Gan Liu-Gen, Xiao Chun-Lan, Shan Zhi-Ling, Yang Rong, Song Hao, Li Liu, Liu Bei-Zhong
1. Central Laboratory of Yong-chuan Hospital, Chongqing Medical University, Chongqing, 402160, China; 2. Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China.
2. Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China.
Int J Med Sci. 2016 Jun 29;13(7):517-23. doi: 10.7150/ijms.15507. eCollection 2016.
To investigate the effect of LG100268 (LG268) on cell proliferation and apoptosis in NB4 cells.
NB4 cells were treated with LG268 for 24 h or 48 h. The effect of LG268 on cell proliferation was assessed by the CCK-8 assay and colony-forming assay. Apoptosis and cell cycle were evaluated by flow cytometry. The protein expression levels of Survivin, PARP, c-Myc, cyclin D1, ERK, p-ERK, p38 MAPK, and p- p38 MAPK were detected by western blot.
We found that LG268 inhibited the proliferation of NB4 cells in a dose-dependent manner. Flow cytometry analysis showed that LG268 accelerated apoptosis in NB4 cells in a time- dependent manner and that LG268 treatment led to cell cycle arrest at G0/G1 phase. Moreover, LG268 significantly decreased the protein levels of Survivin, c-Myc, and cyclinD1. Cleaved PARP was observed in the LG268 treatment group but not in the control group. In addition, LG268 increased the phosphorylation level of p38 MAPK and decreased the phosphorylation level of ERK.
LG268 inhibited cell proliferation and promoted cell apoptosis in NB4 cells.
研究LG100268（LG268）对NB4细胞增殖和凋亡的影响。
用LG268处理NB4细胞24小时或48小时。通过CCK-8法和集落形成试验评估LG268对细胞增殖的影响。通过流式细胞术评估凋亡和细胞周期。通过蛋白质印迹法检测Survivin、PARP、c-Myc、细胞周期蛋白D1、ERK、p-ERK、p38丝裂原活化蛋白激酶（p38 MAPK）和磷酸化p38 MAPK（p-p38 MAPK）的蛋白表达水平。
我们发现LG268以剂量依赖性方式抑制NB4细胞的增殖。流式细胞术分析表明，LG268以时间依赖性方式加速NB4细胞的凋亡，并且LG268处理导致细胞周期停滞在G0/G1期。此外，LG268显著降低Survivin、c-Myc和细胞周期蛋白D1的蛋白水平。在LG268处理组中观察到裂解的PARP，但在对照组中未观察到。此外，LG268增加p38 MAPK的磷酸化水平并降低ERK的磷酸化水平。
LG268抑制NB4细胞的增殖并促进其凋亡。
