Muzila Mbaki, Rumpunen Kimmo, Wright Helen, Roberts Helen, Grant Melissa, Nybom Hilde, Sehic Jasna, Ekholm Anders, Widén Cecilia
Department of Plant Breeding, Swedish University of Agricultural Sciences, Balsgård, Fjälkestadsvägen 459, 291 94 Kristianstad, Sweden; Biological Sciences, University of Botswana, Private Bag UB 00704, Gaborone, Botswana.
Department of Plant Breeding, Swedish University of Agricultural Sciences, Balsgård, Fjälkestadsvägen 459, 291 94 Kristianstad, Sweden.
Oxid Med Cell Longev. 2016;2016:3841803. doi: 10.1155/2016/3841803. Epub 2016 Jun 27.
Harpagophytum, Devil's Claw, is a genus of tuberiferous xerophytic plants native to southern Africa. Some of the taxa are appreciated for their medicinal effects and have been traditionally used to relieve symptoms of inflammation. The objectives of this pilot study were to investigate the antioxidant capacity and the content of total phenols, verbascoside, isoverbascoside, and selected iridoids, as well as to investigate the capacity of various Harpagophytum taxa in suppressing respiratory burst in terms of reactive oxygen species produced by human neutrophils challenged with phorbol myristate acetate (PMA), opsonised Staphylococcus aureus, and Fusobacterium nucleatum. Harpagophytum plants were classified into different taxa according to morphology, and DNA analysis was used to confirm the classification. A putative new variety of H. procumbens showed the highest degree of antioxidative capacity. Using PMA, three Harpagophytum taxa showed anti-inflammatory effects with regard to the PBS control. A putative hybrid between H. procumbens and H. zeyheri in contrast showed proinflammatory effect on the response of neutrophils to F. nucleatum in comparison with treatment with vehicle control. Harpagophytum taxa were biochemically very variable and the response in suppressing respiratory burst differed. Further studies with larger number of subjects are needed to corroborate anti-inflammatory effects of different taxa of Harpagophytum.
钩果草，即魔鬼爪，是一种原产于非洲南部的块茎旱生植物属。其中一些分类群因其药用功效而受到重视，传统上被用于缓解炎症症状。本初步研究的目的是调查其抗氧化能力以及总酚、毛蕊花糖苷、异毛蕊花糖苷和选定环烯醚萜类化合物的含量，同时研究不同钩果草分类群在抑制由佛波酯（PMA）、调理金黄色葡萄球菌和具核梭杆菌刺激人中性粒细胞产生的活性氧方面的呼吸爆发能力。根据形态学将钩果草植物分为不同分类群，并使用DNA分析来确认分类。一种推定的平卧钩果草新品种表现出最高程度的抗氧化能力。使用PMA时，与PBS对照相比，三个钩果草分类群显示出抗炎作用。相比之下，一种推定的平卧钩果草与泽氏钩果草的杂交种在中性粒细胞对具核梭杆菌的反应方面与载体对照处理相比显示出促炎作用。钩果草分类群在生化方面差异很大，其在抑制呼吸爆发方面的反应也不同。需要对更多受试者进行进一步研究，以证实不同钩果草分类群的抗炎作用。
