• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

木犀草素通过清除活性氧来减少紫外线A诱导的人皮肤成纤维细胞自噬。

Luteolin decreases the UVA‑induced autophagy of human skin fibroblasts by scavenging ROS.

作者信息

Yan Miaomiao, Liu Zhongrong, Yang Huilan, Li Cuihua, Chen Hulin, Liu Yan, Zhao Minling, Zhu Yingjie

机构信息

Department of Dermatology, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China.

出版信息

Mol Med Rep. 2016 Sep;14(3):1986-92. doi: 10.3892/mmr.2016.5517. Epub 2016 Jul 13.

DOI:10.3892/mmr.2016.5517
PMID:27430964
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4991762/
Abstract

Luteolin (LUT) is a flavone, which is universally present as a constituent of traditional Chinese herbs, and certain vegetables and spices, and has been demonstrated to exhibit potent radical scavenging and cytoprotective properties. Although LUT has various beneficial effects on health, the effects of LUT on the protection of skin remain to be fully elucidated. The present study investigated whether LUT can protect human skin fibroblasts (HSFs) from ultraviolet (UV) A irradiation. It was found that, following exposure to different doses of UVA irradiation, the HSFs exhibited autophagy, as observed by fluorescence and transmission electron microscopy, and reactive oxygen species (ROS) bursts, analyzed by flow cytometry, to differing degrees. Following incubation with micromolar concentrations of LUT, ROS production decreased and autophagy gradually declined. In addition, the expression of hypoxia‑inducible factor‑1α and the classical autophagy‑associated proteins, LC3 and Beclin 1 were observed by western blotting. Western blot analysis showed that the expression levels of HIF‑1α, LC3‑II and Beclin 1 gradually decreased in the UVA‑irradiated HSFs following treatment with LUT. These data indicated that UVA‑induced autophagy was mediated by ROS, suggesting the possibility of resistance against UV by certain natural antioxidants, including LUT.

摘要

木犀草素（LUT）是一种黄酮类化合物，普遍存在于传统中草药、某些蔬菜和香料中，并且已被证明具有强大的自由基清除和细胞保护特性。尽管LUT对健康有多种有益作用，但其对皮肤保护的作用仍有待充分阐明。本研究调查了LUT是否能保护人皮肤成纤维细胞（HSF）免受紫外线（UV）A照射。结果发现，在暴露于不同剂量的UVA照射后，通过荧光和透射电子显微镜观察到HSF表现出自噬，通过流式细胞术分析发现其活性氧（ROS）爆发程度不同。在用微摩尔浓度的LUT孵育后，ROS产生减少，自噬逐渐下降。此外，通过蛋白质印迹法观察缺氧诱导因子-1α以及经典的自噬相关蛋白LC3和Beclin 1的表达。蛋白质印迹分析表明，在用LUT处理后，UVA照射的HSF中HIF-1α、LC3-II和Beclin 1的表达水平逐渐降低。这些数据表明，UVA诱导的自噬由ROS介导，这提示包括LUT在内的某些天然抗氧化剂具有抵抗紫外线的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c2/4991762/0e072c93220a/MMR-14-03-1986-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c2/4991762/36afddc6d339/MMR-14-03-1986-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c2/4991762/81378c565ea0/MMR-14-03-1986-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c2/4991762/9371507a0db3/MMR-14-03-1986-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c2/4991762/f8655bd89474/MMR-14-03-1986-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c2/4991762/0fedcc1f1fd9/MMR-14-03-1986-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c2/4991762/0e072c93220a/MMR-14-03-1986-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c2/4991762/36afddc6d339/MMR-14-03-1986-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c2/4991762/81378c565ea0/MMR-14-03-1986-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c2/4991762/9371507a0db3/MMR-14-03-1986-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c2/4991762/f8655bd89474/MMR-14-03-1986-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c2/4991762/0fedcc1f1fd9/MMR-14-03-1986-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c2/4991762/0e072c93220a/MMR-14-03-1986-g05.jpg

相似文献

1
Luteolin decreases the UVA‑induced autophagy of human skin fibroblasts by scavenging ROS.木犀草素通过清除活性氧来减少紫外线A诱导的人皮肤成纤维细胞自噬。
Mol Med Rep. 2016 Sep;14(3):1986-92. doi: 10.3892/mmr.2016.5517. Epub 2016 Jul 13.
2
ROS-AKT-mTOR axis mediates autophagy of human umbilical vein endothelial cells induced by cooking oil fumes-derived fine particulate matters in vitro.ROS-AKT-mTOR 轴介导烹调油烟细颗粒物诱导人脐静脉内皮细胞自噬的体外研究。
Free Radic Biol Med. 2017 Dec;113:452-460. doi: 10.1016/j.freeradbiomed.2017.10.386. Epub 2017 Oct 28.
3
The flavonoid luteolin enhances doxorubicin-induced autophagy in human osteosarcoma U2OS cells.类黄酮木犀草素增强阿霉素诱导的人骨肉瘤U2OS细胞自噬。
Int J Clin Exp Med. 2015 Sep 15;8(9):15190-7. eCollection 2015.
4
Luteolin Promotes Cell Apoptosis by Inducing Autophagy in Hepatocellular Carcinoma.木犀草素通过诱导自噬促进肝癌细胞凋亡。
Cell Physiol Biochem. 2017;43(5):1803-1812. doi: 10.1159/000484066. Epub 2017 Oct 19.
5
Luteolin promotes the sensitivity of cisplatin in ovarian cancer by decreasing PRPA1-medicated autophagy.木犀草素通过降低PRPA1介导的自噬来提高卵巢癌对顺铂的敏感性。
Cell Mol Biol (Noisy-le-grand). 2018 May 15;64(6):17-22.
6
Baicalin protects human skin fibroblasts from ultraviolet A radiation-induced oxidative damage and apoptosis.黄芩素可保护人皮肤成纤维细胞免受紫外线 A 辐射诱导的氧化损伤和凋亡。
Free Radic Res. 2012 Dec;46(12):1458-71. doi: 10.3109/10715762.2012.726355. Epub 2012 Sep 24.
7
Induction of endoplasmic reticulum stress-mediated apoptosis and non-canonical autophagy by luteolin in NCI-H460 lung carcinoma cells.木樨草素诱导 NCI-H460 肺癌细胞内质网应激介导的细胞凋亡和非典型自噬。
Food Chem Toxicol. 2013 Jun;56:100-9. doi: 10.1016/j.fct.2013.02.022. Epub 2013 Feb 20.
8
The photoprotective and antioxidative properties of luteolin are synergistically augmented by tocopherol and ubiquinone.木樨草素的光保护和抗氧化特性通过生育酚和泛醌协同增强。
Planta Med. 2013 Jul;79(11):963-5. doi: 10.1055/s-0032-1328716. Epub 2013 Jul 9.
9
Zeolite encapsulation decreases TiO2-photosensitized ROS generation in cultured human skin fibroblasts.沸石包封可减少培养的人皮肤成纤维细胞中二氧化钛光敏化活性氧的生成。
Photochem Photobiol. 2006 Jan-Feb;82(1):5-12. doi: 10.1562/2005-05-29-RA-551.
10
Luteolin Attenuates Foam Cell Formation and Apoptosis in Ox-LDL-Stimulated Macrophages by Enhancing Autophagy.木犀草素通过增强自噬减轻氧化低密度脂蛋白刺激的巨噬细胞中泡沫细胞的形成和凋亡。
Cell Physiol Biochem. 2016;39(5):2065-2076. doi: 10.1159/000447902. Epub 2016 Oct 31.

引用本文的文献

1
Luteolin targets MKK4 to attenuate particulate matter-induced MMP-1 and inflammation in human keratinocytes.木犀草素靶向MKK4以减轻颗粒物诱导的人角质形成细胞中的MMP-1和炎症反应。
Sci Rep. 2025 May 15;15(1):16848. doi: 10.1038/s41598-025-01090-3.
2
Promotion of cellular differentiation and DNA repair potential in brain cancer cells by Clitoria ternatea L. with rasayana properties in vitro.具有rasayana特性的蝶豆在体外促进脑癌细胞的细胞分化和DNA修复潜力。
J Ayurveda Integr Med. 2025 Jan-Feb;16(1):101050. doi: 10.1016/j.jaim.2024.101050. Epub 2025 Jan 10.
3
Oral intake of bucillamine, carvedilol, metformin, or phenformin does not protect against UVR-induced squamous cell carcinomas in hairless mice.

本文引用的文献

1
Autophagy-related gene 7 (ATG7) and reactive oxygen species/extracellular signal-regulated kinase regulate tetrandrine-induced autophagy in human hepatocellular carcinoma.自噬相关基因 7（ATG7）和活性氧/细胞外信号调节激酶调节汉防己甲素诱导的人肝癌细胞自噬。
J Biol Chem. 2012 Oct 12;287(42):35576-35588. doi: 10.1074/jbc.M112.370585. Epub 2012 Aug 27.
2
The flavonoid luteolin increases the resistance of normal, but not malignant keratinocytes, against UVB-induced apoptosis.类黄酮木犀草素增加正常角质细胞（而非恶性角质细胞）对 UVB 诱导的细胞凋亡的抵抗力。
J Invest Dermatol. 2010 Sep;130(9):2277-85. doi: 10.1038/jid.2010.124. Epub 2010 May 13.
3
口服布西拉明、卡维地洛、二甲双胍或苯乙双胍不能预防无毛小鼠紫外线辐射诱导的鳞状细胞癌。
Photochem Photobiol Sci. 2024 Mar;23(3):517-526. doi: 10.1007/s43630-024-00535-4. Epub 2024 Feb 9.
4
Luteolin: a flavonoid with a multifaceted anticancer potential.木犀草素：一种具有多方面抗癌潜力的类黄酮。
Cancer Cell Int. 2022 Dec 8;22(1):386. doi: 10.1186/s12935-022-02808-3.
5
Folic acid-modified ROS-responsive nanoparticles encapsulating luteolin for targeted breast cancer treatment.叶酸修饰的 ROS 响应性纳米粒子包载木犀草素用于靶向乳腺癌治疗。
Drug Deliv. 2021 Dec;28(1):1695-1708. doi: 10.1080/10717544.2021.1963351.
6
Autophagy: Multiple Mechanisms to Protect Skin from Ultraviolet Radiation-Driven Photoaging.自噬：多种机制保护皮肤免受紫外线驱动的光老化。
Oxid Med Cell Longev. 2019 Dec 13;2019:8135985. doi: 10.1155/2019/8135985. eCollection 2019.
7
Silymarin: Friend or Foe of UV Exposed Keratinocytes?水飞蓟素：紫外线暴露的角质形成细胞的朋友还是敌人？
Molecules. 2019 Apr 26;24(9):1652. doi: 10.3390/molecules24091652.
8
More Than Skin Deep: Autophagy Is Vital for Skin Barrier Function.不止于皮肤表面：自噬对皮肤屏障功能至关重要。
Front Immunol. 2018 Jun 25;9:1376. doi: 10.3389/fimmu.2018.01376. eCollection 2018.
9
Liposome encapsulated luteolin showed enhanced antitumor efficacy to colorectal carcinoma.脂质体包封的木犀草素显示出增强的抗肿瘤功效，可用于结直肠癌。
Mol Med Rep. 2018 Feb;17(2):2456-2464. doi: 10.3892/mmr.2017.8185. Epub 2017 Nov 29.
3-(4-(Benzo[d]thiazol-2-yl)-1-phenyl-1H-pyrazol-3-yl) phenyl acetate induced Hep G2 cell apoptosis through a ROS-mediated pathway.
3-(4-(苯并[d]噻唑-2-基)-1-苯基-1H-吡唑-3-基)苯基乙酸酯通过 ROS 介导的途径诱导 Hep G2 细胞凋亡。
Chem Biol Interact. 2010 Feb 12;183(3):341-8. doi: 10.1016/j.cbi.2009.12.008. Epub 2009 Dec 16.
4
Distribution and biological activities of the flavonoid luteolin.黄酮类化合物木犀草素的分布及生物活性
Mini Rev Med Chem. 2009 Jan;9(1):31-59. doi: 10.2174/138955709787001712.
5
Anti-carcinogenic effects of the flavonoid luteolin.黄酮类化合物木犀草素的抗癌作用。
Molecules. 2008 Oct 22;13(10):2628-2651. doi: 10.3390/molecules13102628.
6
Apoptosis-inducing effects of two anthraquinones from Hedyotis diffusa WILLD.白花蛇舌草中两种蒽醌的诱导凋亡作用
Biol Pharm Bull. 2008 Jun;31(6):1075-8. doi: 10.1248/bpb.31.1075.
7
Attenuation of doxorubicin-induced contractile and mitochondrial dysfunction in mouse heart by cellular glutathione peroxidase.细胞谷胱甘肽过氧化物酶减轻阿霉素诱导的小鼠心脏收缩和线粒体功能障碍
Free Radic Biol Med. 2006 Jul 1;41(1):46-55. doi: 10.1016/j.freeradbiomed.2006.02.024. Epub 2006 Apr 4.
8
Leishmania donovani: intracellular ATP level regulates apoptosis-like death in luteolin induced dyskinetoplastid cells.杜氏利什曼原虫：细胞内ATP水平调节木犀草素诱导的动基体发育异常细胞中的凋亡样死亡。
Exp Parasitol. 2006 Nov;114(3):204-14. doi: 10.1016/j.exppara.2006.03.013. Epub 2006 May 16.
9
Luteolin and chrysin differentially inhibit cyclooxygenase-2 expression and scavenge reactive oxygen species but similarly inhibit prostaglandin-E2 formation in RAW 264.7 cells.木犀草素和白杨素对RAW 264.7细胞中环氧化酶-2的表达有不同程度的抑制作用，对活性氧有清除作用，但对前列腺素-E2的生成有相似的抑制作用。
J Nutr. 2006 Jun;136(6):1517-21. doi: 10.1093/jn/136.6.1517.
10
Chemopreventive potential of luteolin during colon carcinogenesis induced by 1,2-dimethylhydrazine.木犀草素在1,2 - 二甲基肼诱导的结肠癌发生过程中的化学预防潜力。
Ital J Biochem. 2005 Sep-Dec;54(3-4):268-75.