Department of Dermatology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, 4032 Debrecen, Hungary.
Department of Pharmaceutical Technology, University of Debrecen, Nagyerdei körút 98, 4032 Debrecen, Hungary.
Molecules. 2019 Apr 26;24(9):1652. doi: 10.3390/molecules24091652.
The application of natural plant extracts in UV-protection is popular and intensively studied. Silymarin (from ), a naturally occurring polyphenol, has recently received attention due to its antioxidant, anti-inflammatory and anti-apoptotic effects. However, its role in the UV-mediated keratinocyte cell response is still controversial. In this study, we investigated the effects of extracts with different origins and formulations on UVA-exposed HaCaT keratinocytes in vitro. Our results show, that silymarin treatment caused an inverse dose-dependent photosensitivity relationship (at higher doses, a decrease in cell viability and ROS production) after UVA exposure. The attenuation of the UVA-induced ROS generation after silymarin treatment was also observed. Moreover, silymarin pre-treatment increased the cyclobutane pyrimidine dimer photolesions in keratinocytes after UVA exposure. These results indicated the dual role of silymarin in UVA-exposed keratinocytes. It scavenges ROS but still induces phototoxicity. Based on our results dermatological applications of silymarin and related compounds should be considered very carefully.
天然植物提取物在防晒中的应用很受欢迎，并且受到了深入研究。水飞蓟素（来自 ）是一种天然存在的多酚，由于其抗氧化、抗炎和抗细胞凋亡作用，最近受到了关注。然而，它在 UV 介导的角质形成细胞反应中的作用仍存在争议。在这项研究中，我们研究了不同来源和配方的 提取物对体外 UVA 暴露的 HaCaT 角质形成细胞的影响。我们的结果表明，水飞蓟素处理后会导致 UVA 暴露后的反向剂量依赖性光敏感性关系（较高剂量时，细胞活力和 ROS 产生减少）。在用水飞蓟素处理后，UVA 诱导的 ROS 生成也减弱了。此外，水飞蓟素预处理增加了 UVA 暴露后角质形成细胞中环丁烷嘧啶二聚体光损伤。这些结果表明水飞蓟素在 UVA 暴露的角质形成细胞中具有双重作用。它清除了 ROS，但仍会诱导光毒性。基于我们的结果，水飞蓟素和相关化合物的皮肤科应用应谨慎考虑。
