Zhang Baoliang, Yu Xin, Xia Hong
Southern Medical University Guangzhou, China ; Department of Orthopedics, Guangzhou General Hospital of Guangzhou Military Command Guangzhou, China ; Department of Spine Surgery, Yongzhou Central Hospital Yongzhou, China.
Department of Orthopedics, Tai'an City Central Hospital Tai'an, China.
Int J Clin Exp Med. 2015 Sep 15;8(9):15190-7. eCollection 2015.
Luteolin (LUT), a flavone, which is universally present as constituent of medicinal plants as well as some vegetables and spices, has been demonstrated display specific anti-carcinogenic effects. However, the mechanisms by which LUT inhibits human osteosarcoma growth remain unknown. The effects of LUT on cell growth in human osteosarcoma U2OS cells were measured by MTT assay and flowcytometry. The effects of LUT on morphological markers of autophagy in U2OS were analyzed by fluorescence microscopy and electron microscopy. Autophagic markers, beclin1 and LC3 were detected by western blotting. Here, we found that LUT induced autophagy in U2OS and acted as an enhancer to sensitize doxorubicin (DOX)-mediated autophagy signaling. The combined treatment of LUT and DOX greatly decreases the growth of U2OS, showing synergistic cytotoxicity. Our results indicate that LUT in combination with DOX maybe a novel strategy for the treatment of human osteosarcoma.
木犀草素（LUT）是一种黄酮类化合物，作为药用植物以及一些蔬菜和香料的成分普遍存在，已被证明具有特定的抗癌作用。然而，LUT抑制人骨肉瘤生长的机制尚不清楚。通过MTT法和流式细胞术检测LUT对人骨肉瘤U2OS细胞生长的影响。通过荧光显微镜和电子显微镜分析LUT对U2OS细胞自噬形态学标志物的影响。通过蛋白质免疫印迹法检测自噬标志物beclin1和LC3。在此，我们发现LUT诱导U2OS细胞自噬，并作为增强剂使阿霉素（DOX）介导的自噬信号敏感化。LUT和DOX联合治疗大大降低了U2OS细胞的生长，显示出协同细胞毒性。我们的结果表明，LUT与DOX联合使用可能是治疗人骨肉瘤的一种新策略。
