Del Carmen Terrón María, González-Camacho Fernando, González Luis Miguel, Luque Daniel, Montero Estrella
Servicio de Microscopia Electrónica y Confocal, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km 2.2, 28220 Majadahonda, Madrid, Spain.
Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahoda-Pozuelo Km 2.2, 28220 Majadahonda, Madrid, Spain.
Ticks Tick Borne Dis. 2016 Oct;7(6):1274-1279. doi: 10.1016/j.ttbdis.2016.07.001. Epub 2016 Jul 5.
The invasive form of the apicomplexan parasite Babesia divergens, the free merozoite, invades the erythrocytes of host vertebrates, leading to significant pathology. Although invasion is an active process critical for parasite survival, it is not yet entirely understood. Using techniques to isolate the viable free merozoite, as well as electron microscopy, we undertook a detailed morphological study and explored the sub-cellular structure of the invasive B. divergens free merozoite after it had left the host cell. We examined characteristic apicomplexan features such as the apicoplast, the inner and discontinuous double membrane complex, and the apical complex; some aspects of erythrocyte entry by B. divergens were also defined by electron microscopy. This study adds to our understanding of B. divergens free merozoites and their invasion of human erythrocytes.
顶复门寄生虫分歧巴贝斯虫（Babesia divergens）的侵袭性形式，即游离裂殖子，会侵入宿主脊椎动物的红细胞，导致严重病变。尽管侵袭是对寄生虫生存至关重要的一个活跃过程，但目前尚未完全了解。我们运用分离活游离裂殖子的技术以及电子显微镜，进行了详细的形态学研究，并在其离开宿主细胞后探究了侵袭性分歧巴贝斯虫游离裂殖子的亚细胞结构。我们检查了顶复门的特征结构，如质体、内部不连续的双层膜复合体以及顶端复合体；分歧巴贝斯虫侵入红细胞的一些方面也通过电子显微镜得以明确。这项研究增进了我们对分歧巴贝斯虫游离裂殖子及其对人类红细胞侵袭的理解。
