Tan Zhengyu, Zhou Jun, Chen Houyang, Zou Qianxing, Weng Shiqi, Luo Tao, Tang Yuxin
Department of Urology, The Third Xiangya Hospital, The Central South University, China.
J Toxicol Sci. 2016;41(4):543-9. doi: 10.2131/jts.41.543.
The herbicide 2,4-Dichlorophenoxyacetic acid (2,4-D) is globally used in agriculture and has been linked to human sperm abnormalities in vivo. However, its effects on ejaculated human spermatozoa in vitro have not been characterized. Therefore, we examined the effects of 2,4-D on the functions of ejaculated human spermatozoa in vitro, including: sperm motility, the ability to move through a viscous medium, capacitation, and the acrosome reaction. Different doses of 2,4-D (10 nM, 100 nM, 1 µM, 10 µM, 100 µM, and 200 µM) were applied to human spermatozoa prepared from normal fresh semen samples. The results indicated that 2,4-D did not affect the viability, capacitation, or spontaneous acrosome reactions of human spermatozoa, but it dose-dependently inhibited the total motility, progressive motility, ability to penetrate viscous medium, and progesterone-induced capacitation and acrosome reaction rates. These results suggest that exposure to 2,4-D and its accumulation in the seminal plasma and follicular fluid might increase the risk of infertility. Our findings provide new insights for understanding the male reproductive toxicity of 2,4-D.
除草剂2,4-二氯苯氧乙酸（2,4-D）在全球农业中广泛使用，并且已被证明与体内人类精子异常有关。然而，其对体外射出的人类精子的影响尚未得到明确阐述。因此，我们研究了2,4-D对体外射出的人类精子功能的影响，包括：精子活力、在粘性介质中移动的能力、获能以及顶体反应。将不同剂量的2,4-D（10 nM、100 nM、1 µM、10 µM、100 µM和200 µM）应用于从正常新鲜精液样本中制备的人类精子。结果表明，2,4-D不影响人类精子的活力、获能或自发顶体反应，但它会剂量依赖性地抑制总活力、前向运动能力、穿透粘性介质的能力以及孕酮诱导的获能和顶体反应率。这些结果表明，接触2,4-D及其在精浆和卵泡液中的积累可能会增加不孕风险。我们的研究结果为理解2,4-D的男性生殖毒性提供了新的见解。
