Luo Tao, Li Na, He Yuan-qiao, Weng Shi-qi, Wang Tao, Zou Qian-xing, Zeng Xu-hui
Institute of Life Science, Nanchang University, Nanchang, Jiangxi 330031, PR China.
Institute of Life Science, Nanchang University, Nanchang, Jiangxi 330031, PR China.
Reprod Toxicol. 2015 Jan;51:14-21. doi: 10.1016/j.reprotox.2014.11.007. Epub 2014 Nov 22.
Emodin, a bioactive anthraquinone widely used in Chinese traditional medicine, disrupts mouse testicular gene expression in vivo. In this study, we investigated the toxicity of emodin to human sperm in vitro. Different doses of emodin (25, 50, 100, 200 and 400μM) were applied to ejaculated human sperm. The results indicated that 100, 200 and 400μM emodin significantly inhibited the total motility, progressive motility and linear velocity of human sperm. In addition, sperm's ability to penetrate viscous medium together with progesterone induced capacitation and acrosome reaction was also adversely affected by emodin. In contrast, emodin did not affect sperm viability. Furthermore, intracellular Ca(2+) concentration ([Ca(2+)]i) and tyrosine phosphorylation, which serve as key regulators of sperm function, were dose-dependently reduced by emodin (50-400μM). These results suggest that emodin inhibits human sperm functions by reducing sperm [Ca(2+)]i and suppressing tyrosine phosphorylation in vitro.
大黄素是一种广泛应用于中国传统医学的生物活性蒽醌，它在体内会破坏小鼠睾丸基因表达。在本研究中，我们调查了大黄素在体外对人类精子的毒性。将不同剂量的大黄素（25、50、100、200和400μM）应用于射出的人类精子。结果表明，100、200和400μM大黄素显著抑制了人类精子的总活力、前向运动能力和直线速度。此外，大黄素还对精子穿透粘性介质以及孕酮诱导的获能和顶体反应的能力产生了不利影响。相比之下，大黄素不影响精子活力。此外，作为精子功能关键调节因子的细胞内Ca(2+)浓度（[Ca(2+)]i）和酪氨酸磷酸化，被大黄素（50 - 400μM）呈剂量依赖性降低。这些结果表明，大黄素在体外通过降低精子[Ca(2+)]i和抑制酪氨酸磷酸化来抑制人类精子功能。
