2016年世界卫生组织淋病奈瑟菌新型参考菌株用于实验室检测的全球质量保证：表型、基因及参考基因组特征分析
The novel 2016 WHO Neisseria gonorrhoeae reference strains for global quality assurance of laboratory investigations: phenotypic, genetic and reference genome characterization.
作者信息
Unemo Magnus, Golparian Daniel, Sánchez-Busó Leonor, Grad Yonatan, Jacobsson Susanne, Ohnishi Makoto, Lahra Monica M, Limnios Athena, Sikora Aleksandra E, Wi Teodora, Harris Simon R
机构信息
WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden
WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
出版信息
J Antimicrob Chemother. 2016 Nov;71(11):3096-3108. doi: 10.1093/jac/dkw288. Epub 2016 Jul 17.
OBJECTIVES
Gonorrhoea and MDR Neisseria gonorrhoeae remain public health concerns globally. Enhanced, quality-assured, gonococcal antimicrobial resistance (AMR) surveillance is essential worldwide. The WHO global Gonococcal Antimicrobial Surveillance Programme (GASP) was relaunched in 2009. We describe the phenotypic, genetic and reference genome characteristics of the 2016 WHO gonococcal reference strains intended for quality assurance in the WHO global GASP, other GASPs, diagnostics and research worldwide.
METHODS
The 2016 WHO reference strains (n = 14) constitute the eight 2008 WHO reference strains and six novel strains. The novel strains represent low-level to high-level cephalosporin resistance, high-level azithromycin resistance and a porA mutant. All strains were comprehensively characterized for antibiogram (n = 23), serovar, prolyliminopeptidase, plasmid types, molecular AMR determinants, N. gonorrhoeae multiantigen sequence typing STs and MLST STs. Complete reference genomes were produced using single-molecule PacBio sequencing.
RESULTS
The reference strains represented all available phenotypes, susceptible and resistant, to antimicrobials previously and currently used or considered for future use in gonorrhoea treatment. All corresponding resistance genotypes and molecular epidemiological types were described. Fully characterized, annotated and finished references genomes (n = 14) were presented.
CONCLUSIONS
The 2016 WHO gonococcal reference strains are intended for internal and external quality assurance and quality control in laboratory investigations, particularly in the WHO global GASP and other GASPs, but also in phenotypic (e.g. culture, species determination) and molecular diagnostics, molecular AMR detection, molecular epidemiology and as fully characterized, annotated and finished reference genomes in WGS analysis, transcriptomics, proteomics and other molecular technologies and data analysis.
目的
淋病和耐多药淋病奈瑟菌仍是全球公共卫生关注的问题。在全球范围内，加强有质量保证的淋球菌抗菌药物耐药性（AMR）监测至关重要。世界卫生组织全球淋球菌抗菌药物监测计划（GASP）于2009年重新启动。我们描述了2016年世界卫生组织淋球菌参考菌株的表型、遗传和参考基因组特征，这些菌株用于世界卫生组织全球GASP、其他GASP、全球诊断和研究中的质量保证。
方法
2016年世界卫生组织参考菌株（n = 14）包括8株2008年世界卫生组织参考菌株和6株新菌株。新菌株代表低水平至高水平头孢菌素耐药、高水平阿奇霉素耐药和一个孔蛋白A（porA）突变体。对所有菌株进行了全面的特征分析，包括抗菌谱（n = 23）、血清型、脯氨酰内肽酶、质粒类型、分子AMR决定因素、淋病奈瑟菌多抗原序列分型（NG-MAST）STs和多位点序列分型（MLST）STs。使用单分子PacBio测序产生完整的参考基因组。
结果
参考菌株代表了以前和目前用于淋病治疗或考虑未来使用的抗菌药物的所有可用表型，包括敏感和耐药。描述了所有相应的耐药基因型和分子流行病学类型。展示了经过全面表征、注释和完成的参考基因组（n = 14）。
结论
2016年世界卫生组织淋球菌参考菌株旨在用于实验室调查中的内部和外部质量保证及质量控制，特别是在世界卫生组织全球GASP和其他GASP中，但也用于表型（如培养、菌种鉴定）和分子诊断、分子AMR检测、分子流行病学，以及作为全基因组测序（WGS）分析、转录组学、蛋白质组学和其他分子技术及数据分析中经过全面表征、注释和完成的参考基因组。
Zotero 插件