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2008年世界卫生组织淋病奈瑟菌参考菌株组的表型和基因特征,旨在用于公共卫生目的的淋球菌抗菌药物耐药性监测的全球质量保证和质量控制。

Phenotypic and genetic characterization of the 2008 WHO Neisseria gonorrhoeae reference strain panel intended for global quality assurance and quality control of gonococcal antimicrobial resistance surveillance for public health purposes.

作者信息

Unemo Magnus, Fasth Oskar, Fredlund Hans, Limnios Athena, Tapsall John

机构信息

Department of Clinical Microbiology, Orebro University Hospital, Sweden.

出版信息

J Antimicrob Chemother. 2009 Jun;63(6):1142-51. doi: 10.1093/jac/dkp098. Epub 2009 Mar 24.

Abstract

OBJECTIVES

Emergence and spread of antimicrobial resistance (AMR) in Neisseria gonorrhoeae remain a major global problem and expanded, but valid, AMR surveillance is crucial for public health purposes. The World Health Organization (WHO) Collaborating Centre in Sydney, Australia, continually evaluates N. gonorrhoeae strains used in quality control and assurance aspects of the national, WHO regional and international programmes for AMR surveillance it conducts. Here we phenotypically and genetically characterized the 2008 WHO N. gonorrhoeae reference panel, widely used under existing WHO AMR surveillance protocols.

MATERIALS AND METHODS

The eight N. gonorrhoeae WHO reference strains were phenotypically characterized by antibiogram, auxotype, serovar and prolyliminopeptidase screening; and genetically with regard to resistance plasmid types, polymorphisms in divergent genetic resistance-mediating loci (n = 9), porB sequencing and N. gonorrhoeae multi-antigen sequence typing.

RESULTS

The 2008 WHO reference strains represented all the important susceptible and resistant phenotypes, including corresponding resistance genotypes, and the range of resistances currently seen for relevant antimicrobials. Several pertinent additional phenotypic and genotypic markers, for example, epidemiological markers, were also determined.

CONCLUSIONS

The 2008 WHO N. gonorrhoeae reference strain panel was extensively characterized, which is crucial for the expansion of gonococcal AMR surveillance nationally and internationally. The panel is available through WHO sources for quality assurance and quality control aspects of current phenotypic testing protocols, to allow valid comparison of AMR data derived by divergent methods, and also for the control of present and future molecular assays for AMR detection. Additional WHO reference strains can be included as required by the emergence of additional resistant phenotypes and/or genotypes.

摘要

目的

淋病奈瑟菌中抗菌药物耐药性(AMR)的出现和传播仍然是一个重大的全球问题,扩大但有效的AMR监测对于公共卫生目的至关重要。澳大利亚悉尼的世界卫生组织(WHO)合作中心持续评估其开展的国家、WHO区域和国际AMR监测计划在质量控制和保证方面所使用的淋病奈瑟菌菌株。在此,我们对2008年WHO淋病奈瑟菌参考菌株组进行了表型和基因特征分析,该菌株组在现有的WHO AMR监测方案下被广泛使用。

材料与方法

对8株WHO淋病奈瑟菌参考菌株进行了表型特征分析,包括抗菌谱、营养型、血清型和脯氨酰内肽酶筛选;并从耐药质粒类型、不同基因耐药介导位点(n = 9)的多态性、porB测序和淋病奈瑟菌多抗原序列分型方面进行了基因特征分析。

结果

2008年WHO参考菌株代表了所有重要的敏感和耐药表型,包括相应的耐药基因型,以及目前相关抗菌药物的耐药范围。还确定了几个相关的额外表型和基因型标记,例如流行病学标记。

结论

对2008年WHO淋病奈瑟菌参考菌株组进行了广泛的特征分析,这对于在国家和国际上扩大淋球菌AMR监测至关重要。该菌株组可通过WHO来源获取,用于当前表型检测方案的质量保证和质量控制,以便对不同方法得出的AMR数据进行有效比较,也用于控制当前和未来AMR检测的分子检测。可根据出现的额外耐药表型和/或基因型,按要求纳入更多WHO参考菌株。

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