Christov K, Milev A, Todorov V
Bulgarian Medical Academy, National Center of Oncology, Department of Pathology, Sofia.
Cancer. 1989 Aug 1;64(3):673-9. doi: 10.1002/1097-0142(19890801)64:3<673::aid-cncr2820640318>3.0.co;2-d.
The cellular DNA content of 30 benign and 180 malignant breast tumors was analyzed by means of flow cytometry (FCM). All benign tumors exhibited a normal DNA content (diploid), whereas 65% of the malignant tumors showed an abnormal DNA content (aneuploid). The ploidy distribution of malignant tumors was bimodal with an increasing frequency near diploid DNA index (DI), and a second group had a DI ranging from triploid to tetraploid. In estimating the degree of malignancy eight independent histomorphologic and cytologic criteria were introduced. A good correlation was observed between DNA content abnormalities and the grade of differentiation of breast carcinomas. The percentage of S-phase cells of DNA aneuploid cell lines was significantly higher than in the diploid ones. The highly differentiated breast carcinomas (Grade 1) indicated lower S-phase values as compared to the undifferentiated (Grade 3) ones. S-phase values estimated by FCM were about two times higher than the 3H-thymidine labeling index (LI) obtained by an in vitro procedure. The data estimated in this study showed that DNA determinations as an adjunct to conventional histopathologic assessment may provide objective clinically relevant information with respect to the degree of malignancy and prognosis of patients with breast carcinoma.
采用流式细胞术（FCM）分析了30例乳腺良性肿瘤和180例恶性肿瘤的细胞DNA含量。所有良性肿瘤均表现为正常DNA含量（二倍体），而65%的恶性肿瘤显示DNA含量异常（非整倍体）。恶性肿瘤的倍体分布呈双峰型，在接近二倍体DNA指数（DI）处频率增加，另一组的DI范围为三倍体至四倍体。在评估恶性程度时，引入了八个独立的组织形态学和细胞学标准。观察到DNA含量异常与乳腺癌的分化程度之间存在良好的相关性。DNA非整倍体细胞系的S期细胞百分比明显高于二倍体细胞系。高分化乳腺癌（1级）的S期值低于未分化（3级）乳腺癌。通过FCM估计的S期值比通过体外方法获得的3H-胸腺嘧啶核苷标记指数（LI）高约两倍。本研究估计的数据表明，DNA测定作为传统组织病理学评估的辅助手段，可能为乳腺癌患者的恶性程度和预后提供客观的临床相关信息。
