Kaketani K, Saito T, Kobayashi M
Department of Surgery I, Medical College of Oita, Japan.
Cancer. 1989 Aug 15;64(4):887-91. doi: 10.1002/1097-0142(19890815)64:4<887::aid-cncr2820640421>3.0.co;2-1.
Flow cytometric analysis of nuclear DNA content was performed on excised malignant tissue of the esophagus. The DNA ploidy pattern was compared with a variety of histologic parameters and the subsequent clinical course to determine whether or not this pattern is associated with the mode of malignant potentiality. Of the 31 patients, eight had the diploidy DNA pattern and 23 the aneuploid DNA pattern. Tumors with the aneuploidy DNA pattern had a significantly higher frequency of lymph node metastasis than did those with the diploidy DNA pattern (P less than 0.01). Mitotic rates in the aneuploid tumors were significantly higher than was the case in diploid tumors (P less than 0.0005). The incidence of recurrence within 12 months after surgery was higher in patients with aneuploid tumors (83.3%) than in those with diploid ones (16.7%), with a statistical difference (P less than 0.05). Thus, the DNA aneuploidy based on flow cytometry closely correlates with the high frequency of nodal involvement and high mitotic rates, factors generally indicative of the aggressive behavior of the malignant tumors. DNA aneuploidy based on flow cytometric analysis is a pertinent index for determining the highly malignant potential in esophageal carcinoma.
对切除的食管恶性组织进行了细胞核DNA含量的流式细胞术分析。将DNA倍体模式与各种组织学参数以及随后的临床病程进行比较，以确定这种模式是否与恶性潜能模式相关。在31例患者中，8例具有二倍体DNA模式，23例具有非整倍体DNA模式。具有非整倍体DNA模式的肿瘤发生淋巴结转移的频率明显高于具有二倍体DNA模式的肿瘤（P<0.01）。非整倍体肿瘤的有丝分裂率明显高于二倍体肿瘤（P<0.0005）。非整倍体肿瘤患者术后12个月内的复发率（83.3%）高于二倍体肿瘤患者（16.7%），差异有统计学意义（P<0.05）。因此，基于流式细胞术的DNA非整倍体与淋巴结受累的高频率和高有丝分裂率密切相关，这些因素通常表明恶性肿瘤具有侵袭性。基于流式细胞术分析的DNA非整倍体是确定食管癌高恶性潜能的一个相关指标。
