Moran R E, Black M M, Alpert L, Straus M J
Cancer. 1984 Oct 15;54(8):1586-90. doi: 10.1002/1097-0142(19841015)54:8<1586::aid-cncr2820540820>3.0.co;2-9.
DNA ploidy and percent of (%S-phase) S-phase cells were determined from the DNA content distribution of 21 benign and 76 malignant (69 primary, 7 metastatic) breast tumors using flow cytometry. All of the benign tumors were diploid, whereas 89% of the malignant tumors had measurable aneuploidy. Multiple stem-lines were observed in approximately 10% of the malignant tumors. The ploidy distribution of the malignant tumors was bimodal with an increased frequency of tumors with a near diploid DNA index (DI), and a second group with DI ranging from triploid to tetraploid. The percentage of cells in S-phase ranged from less than 1% to 37.4%. DI and %S were significantly higher in poorly differentiated duct carcinomas, medullary carcinomas, and recurrent tumor metastases. DI and %S were also significantly higher in estrogen-receptor-negative tumors. There was no correlation between DI or %S and the extent of axillary nodal metastases. However, within the groups of node-negative and node-positive patients, DI and %S were not randomly distributed but were significantly correlated with degree of nuclear differentiation. Both parameters were higher in poorly differentiated tumors compared with well-differentiated tumors, indicating significant intrastage heterogeneity in tumor ploidy and proliferation characteristics. Determination of the prognostic significance of DI and %S will require a longer follow-up time.
采用流式细胞术，根据21例良性乳腺肿瘤和76例恶性乳腺肿瘤（69例原发性肿瘤，7例转移性肿瘤）的DNA含量分布，测定DNA倍体和S期细胞百分比（%S期）。所有良性肿瘤均为二倍体，而89%的恶性肿瘤存在可检测到的非整倍体。在约10%的恶性肿瘤中观察到多个干细胞系。恶性肿瘤的倍体分布呈双峰型，DNA指数（DI）接近二倍体的肿瘤频率增加，另一组DI范围从三倍体到四倍体。S期细胞百分比范围为小于1%至37.4%。在低分化导管癌、髓样癌和复发性肿瘤转移中，DI和%S显著更高。在雌激素受体阴性肿瘤中，DI和%S也显著更高。DI或%S与腋窝淋巴结转移程度之间无相关性。然而，在淋巴结阴性和阳性患者组中，DI和%S并非随机分布，而是与核分化程度显著相关。与高分化肿瘤相比，低分化肿瘤的这两个参数均更高，表明肿瘤倍体和增殖特征在阶段内存在显著异质性。确定DI和%S的预后意义需要更长的随访时间。
