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Oesophageal basaloid squamous cell carcinoma: a unique clinicopathological entity with telomerase activity as a prognostic indicator.食管基底样鳞状细胞癌：一种独特的临床病理实体，端粒酶活性作为预后指标
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Br J Cancer. 1996 Oct;74(7):1012-6. doi: 10.1038/bjc.1996.482.
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Ploidy as a prognostic indicator in oesophageal squamous carcinoma and its relationship to various histological criteria.
J Pathol. 1989 Sep;159(1):35-41. doi: 10.1002/path.1711590110.
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Flow-cytometric DNA content analysis of oesophageal carcinoma. Comparison between tumour and sequential non-tumour mucosae.食管癌的流式细胞术DNA含量分析。肿瘤组织与连续非肿瘤黏膜的比较。
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Prognostic implication of proliferative markers MIB-1 and PC10 in esophageal squamous cell carcinoma.增殖标志物MIB-1和PC10在食管鳞状细胞癌中的预后意义
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DNA quantification of squamous cell carcinoma of the oesophagus by flow cytometry and cytophotometric image analysis using formalin fixed paraffin embedded tissue.利用福尔马林固定石蜡包埋组织，通过流式细胞术和细胞光度图像分析对食管鳞状细胞癌进行DNA定量分析。
Cytometry. 1992;13(8):886-92. doi: 10.1002/cyto.990130812.
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DNA ploidy, P53 expression, and cellular proliferation in normal epithelium and squamous dysplasia of non-cancerous and cancerous human oesophagi.非癌性和癌性人类食管正常上皮及鳞状发育异常中的DNA倍体、P53表达和细胞增殖
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Composite Pheochromocytoma-Ganglioneuroma of the Adrenal Gland: An Uncommon Entity with Distinctive Clinicopathologic Features.肾上腺复合性嗜铬细胞瘤-神经节瘤：一种具有独特临床病理特征的罕见实体瘤。
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Prognostic implication of proliferative markers MIB-1 and PC10 in esophageal squamous cell carcinoma.增殖标志物MIB-1和PC10在食管鳞状细胞癌中的预后意义
Cancer. 1996 Jan 1;77(1):7-13. doi: 10.1002/(SICI)1097-0142(19960101)77:1<7::AID-CNCR3>3.0.CO;2-N.
2
Prognosis of operable squamous cell carcinoma of the esophagus. Relationship with clinicopathologic features and DNA ploidy.可手术切除的食管鳞状细胞癌的预后。与临床病理特征及DNA倍体的关系。
Cancer. 1993 Jul 1;72(1):20-4. doi: 10.1002/1097-0142(19930701)72:1<20::aid-cncr2820720106>3.0.co;2-j.
3
Prognostic value of DNA ploidy in squamous cell carcinoma of esophagus. Analyzed with improved flow cytometric measurement.食管鳞状细胞癌中DNA倍性的预后价值。采用改良流式细胞术测量进行分析。
Cancer. 1993 Sep 15;72(6):1813-8. doi: 10.1002/1097-0142(19930915)72:6<1813::aid-cncr2820720604>3.0.co;2-r.
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Measurement of nuclear DNA content in histological and cytological specimens: principles and applications.组织学和细胞学标本中核DNA含量的测量：原理与应用
J Pathol. 1994 Apr;172(4):307-12. doi: 10.1002/path.1711720404.
5
Comparison of flow cytometric DNA content analysis of fresh and formalin-fixed paraffin-embedded solid neoplasms.新鲜及福尔马林固定石蜡包埋实体瘤的流式细胞术DNA含量分析比较
Mod Pathol. 1994 Apr;7(3):354-60.
6
p53 overexpression in human esophageal carcinoma: a correlation with tumor DNA ploidy and two parameter flow cytometric study.人食管癌中p53过表达：与肿瘤DNA倍性的相关性及双参数流式细胞术研究
Anticancer Res. 1994 May-Jun;14(3B):1305-12.
7
Update on proliferation-associated antibodies applicable to formalin-fixed paraffin-embedded tissue and their clinical applications.适用于福尔马林固定石蜡包埋组织的增殖相关抗体及其临床应用的最新进展。
Histochem J. 1993 Dec;25(12):843-53.
8
Flow cytometric analysis of DNA heterogeneity in superficial carcinoma of the esophagus.食管浅表癌DNA异质性的流式细胞术分析
Cancer. 1995 Feb 15;75(4):914-9. doi: 10.1002/1097-0142(19950215)75:4<914::aid-cncr2820750404>3.0.co;2-6.
9
Significance of aneuploidy.非整倍体的意义。
Br J Surg. 1994 Oct;81(10):1416-22. doi: 10.1002/bjs.1800811005.
10
Overexpression of the p53 tumor suppressor gene product in esophageal and gastric carcinomas.p53肿瘤抑制基因产物在食管癌和胃癌中的过表达。
Pathol Res Pract. 1994 Dec;190(12):1141-8. doi: 10.1016/S0344-0338(11)80440-1.

流式细胞术在III期食管鳞状细胞癌分析中的应用及其与MIB-1的关联

Use of flow cytometry in the analysis of stage III squamous cell carcinoma of the oesophagus and its association with MIB-1.

作者信息

Lam K Y, Ma L, Law S Y, Cheung L K, Luk T F, Wong J

机构信息

Department of Pathology, University of Hong Kong, Queen Mary Hospital, Hong Kong.

出版信息

J Clin Pathol. 1996 Dec;49(12):975-8. doi: 10.1136/jcp.49.12.975.

DOI:10.1136/jcp.49.12.975
PMID:9038733
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC499644/
Abstract

AIMS

To examine the prognostic and pathobiological importance of DNA content in oesophageal squamous cell carcinomas in Hong Kong Chinese subjects; to evaluate its association with the immunohistochemical proliferative marker MIB-1.

METHODS

Paraffin wax embedded tumour tissue and adjacent normal tissue (control tissue) samples from 45 resected stage III oesophageal squamous cell carcinomas were studied using flow cytometric analysis. The DNA content and the clinicopathological data of these patients were analysed together with the MIB-1 labelling index.

RESULTS

DNA aneuploidy was present in 14 (31%) of the 45 cases. However, the DNA content did not correlate significantly with the age, sex, or survival of the patients, nor the length, location, differentiation and MIB-1 labelling index of the oesophageal carcinomas. The synthetic (S) phase fraction of diploid tumours bore no relation to the patients' survival or MIB-1 score.

CONCLUSIONS

Flow cytometry was not as useful as the MIB-1 labelling index in predicting the biological characteristics of the tumours and the prognosis of patients with oesophageal squamous cell carcinomas. This study does not support the routine use of DNA flow cytometric analysis in oesophageal cancers.

摘要

目的

研究香港华人食管鳞状细胞癌中DNA含量的预后及病理生物学意义；评估其与免疫组化增殖标志物MIB-1的相关性。

方法

采用流式细胞术分析45例切除的Ⅲ期食管鳞状细胞癌石蜡包埋肿瘤组织及相邻正常组织（对照组织）样本。分析这些患者的DNA含量、临床病理数据以及MIB-1标记指数。

结果

45例病例中有14例（31%）存在DNA非整倍体。然而，DNA含量与患者的年龄、性别或生存率，以及食管癌的长度、位置、分化程度和MIB-1标记指数均无显著相关性。二倍体肿瘤的合成（S）期分数与患者的生存率或MIB-1评分无关。

结论

在预测食管鳞状细胞癌患者的肿瘤生物学特征和预后方面，流式细胞术不如MIB-1标记指数有用。本研究不支持在食管癌中常规使用DNA流式细胞术分析。