Zingue Stéphane, Nde Chantal Beatrice Magne, Njamen Dieudonné
J Basic Clin Physiol Pharmacol. 2015 Jan;26(1):53-64. doi: 10.1515/jbcpp-2013-0136.
The use of traditional medicinal plants to treat various diseases is common in Sub-Saharan African countries, including Cameroon. Millettia macrophylla, one of such plants, was previously found to exhibit estrogenic properties in female Wistar rats. However, its effects on the male reproductive system are unknown. Based on literature evidence that phytoestrogens impair male sexual behavior, we aimed at assessing the effect of the extracts of M. macrophylla stem bark on the male reproductive system of Wistar rats.
We evaluated the effects of the dichloromethane (DCM) and methanol (MeOH) soluble fractions of M. macrophylla stem bark on male rat sexual behavior, as well as androgen-dependent parameters for 60 days.
Data showed a significant decrease (p<0.05) in the wiring-touch frequency, mount frequency, intromission frequency, ejaculatory frequency, penile-licking frequency, and computed indices of sexual behavior, throughout the experimental period, as well as a significant increase (p<0.001) in mount, intromission, and ejaculatory latencies as well as post-ejaculatory interval. Moreover, we observed a significant decrease (p<0.05) in the androgen-dependent sexual parameters evaluated. The DCM extract did not induce significant effects on the assessed parameters.
These results suggest that long-term exposure to the estrogenic MeOH fraction of M. macrophylla stem bark negatively alters sexual behavior and spermatogenesis.
在包括喀麦隆在内的撒哈拉以南非洲国家，使用传统药用植物治疗各种疾病很常见。大叶崖豆藤就是其中一种植物，此前发现它在雌性Wistar大鼠中表现出雌激素特性。然而，其对雄性生殖系统的影响尚不清楚。基于文献证据表明植物雌激素会损害雄性性行为，我们旨在评估大叶崖豆藤茎皮提取物对Wistar大鼠雄性生殖系统的影响。
我们评估了大叶崖豆藤茎皮的二氯甲烷（DCM）和甲醇（MeOH）可溶部分对雄性大鼠性行为以及雄激素依赖性参数的影响，为期60天。
数据显示，在整个实验期间，线触频率、爬跨频率、插入频率、射精频率、舔阴茎频率以及性行为计算指数均显著下降（p<0.05），同时爬跨、插入和射精潜伏期以及射精后间隔显著增加（p<0.001）。此外，我们观察到所评估的雄激素依赖性性参数显著下降（p<0.05）。DCM提取物对所评估参数未产生显著影响。
这些结果表明，长期暴露于大叶崖豆藤茎皮的雌激素性MeOH部分会对性行为和精子发生产生负面影响。
