采用制备型高速逆流色谱法从红发夫酵母中分离纯化虾青素。
Separation and purification of astaxanthin from Phaffia rhodozyma by preparative high-speed counter-current chromatography.
作者信息
Du Xiping, Dong Congcong, Wang Kai, Jiang Zedong, Chen Yanhong, Yang Yuanfan, Chen Feng, Ni Hui
机构信息
College of Food and Bioengineering, Jimei University, Xiamen, Fujian 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, Fujian 361021, China; Research Center of Food Biotechnology of Xiamen City, Xiamen, Fujian 361021, China; Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed, Xiamen Southern Ocean Technology Center of China, Xiamen, Fujian 361021, China.
College of Food and Bioengineering, Jimei University, Xiamen, Fujian 361021, China.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Sep 1;1029-1030:191-197. doi: 10.1016/j.jchromb.2016.06.042. Epub 2016 Jun 25.
An effective high-speed counter-current chromatography (HSCCC) method was established for the preparative isolation and purification of astaxanthin from Phaffia rhodozyma. With a two-phase solvent system composed of n-hexane-acetone-ethanol-water (1:1:1:1, v/v/v/v), 100mg crude extract of P. rhodozyma was separated to yield 20.6mg of astaxanthin at 92.0% purity. By further one step silica gel column chromatography, the purity reached 99.0%. The chemical structure of astaxanthin was confirmed by thin layer chromatography (TLC), UV spectroscopy scanning, high performance liquid chromatography with a ZORBAX SB-C18 column and a Waters Nova-pak C18 column, and ESI/MS/MS.
建立了一种有效的高速逆流色谱（HSCCC）方法，用于从红发夫酵母中制备分离和纯化虾青素。采用正己烷 - 丙酮 - 乙醇 - 水（1:1:1:1，v/v/v/v）组成的两相溶剂系统，对100mg红发夫酵母粗提物进行分离，得到纯度为92.0%的虾青素20.6mg。通过进一步一步硅胶柱色谱法，纯度达到99.0%。通过薄层色谱（TLC）、紫外光谱扫描、使用ZORBAX SB - C18柱和Waters Nova - pak C18柱的高效液相色谱以及电喷雾电离串联质谱（ESI/MS/MS）对虾青素的化学结构进行了确证。
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