洗脱-挤压逆流色谱一步法从三种食用褐藻中制备分离岩藻黄质。

One-Step Preparative Separation of Fucoxanthin from Three Edible Brown Algae by Elution-Extrusion Countercurrent Chromatography.

机构信息

Key Laboratory of Aquatic Products Processing of Zhejiang Province, Collaborative Innovation Center of Seafood Deep Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China.

出版信息

Mar Drugs. 2022 Apr 7;20(4):257. doi: 10.3390/md20040257.

DOI:10.3390/md20040257

PMID:35447930

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9024483/

Abstract

A method for batch preparation of fucoxanthin from brown algae was established, which possessed the advantages of high yield and high purity. The ultrasonic-assisted extraction method was used to obtain a crude extract from as the separation sample. Then the crude extract was separated by elution-extrusion countercurrent chromatography. The optimum preparation conditions of fucoxanthin were determined as follows: -hexane-ethanol-water (20:9:11, ::) as a two-phase solvent system, the mobile phase flow rate was 5 mL min, the revolution speed was 800 r min, the loading capacity was 60 mg 10 mL and the temperature was 25 °C. By this method, 12.8 mg fucoxanthin with a purity of 94.72% was obtained from the crude extract of . In addition, when the loading capacity was 50 mg 10 mL, the purity of fucoxanthin reached 96.01%. Two types of by-products, chlorophyll and pheophytin, could also be obtained during the process of separation. This optimal method was further applied to separate fucoxanthin from and , and 6.0 mg and 9.7 mg fucoxanthin with a purity of 96.24% and 92.62% were acquired, respectively. Therefore, it was demonstrated that the preparation method of fucoxanthin established in this study had an applicability to brown algae, which improved the utilization value of raw materials.

摘要

建立了一种从褐藻中批量制备岩藻黄质的方法，该方法具有产率高、纯度高的优点。采用超声辅助提取法从中获得粗提取物作为分离样品。然后，通过洗脱-挤出逆流色谱法对粗提取物进行分离。确定岩藻黄质的最佳制备条件如下：-正己烷-乙醇-水（20:9:11， ::）为两相溶剂体系，流动相流速为 5 mL min，转速为 800 r min，装料量为 60 mg 10 mL，温度为 25°C。通过该方法，从的粗提取物中获得了 12.8 mg 纯度为 94.72%的岩藻黄质。此外，当装料量为 50 mg 10 mL 时，岩藻黄质的纯度达到 96.01%。在分离过程中还可以得到两种副产物，叶绿素和脱镁叶绿素。该最佳方法进一步应用于从和中分离岩藻黄质，分别获得了 6.0 mg 和 9.7 mg 纯度为 96.24%和 92.62%的岩藻黄质。因此，研究表明，本研究建立的岩藻黄质制备方法对褐藻具有适用性，提高了原料的利用价值。