Lamelas Araceli, Ampah Kobina Assan, Aboagye Samuel, Kerber Sarah, Danso Emelia, Asante-Poku Adwoa, Asare Prince, Parkhill Julian, Harris Simon R, Pluschke Gerd, Yeboah-Manu Dorothy, Röltgen Katharina
Swiss Tropical and Public Health Institute, Basel, Switzerland.
University of Basel, Basel, Switzerland.
PLoS Negl Trop Dis. 2016 Jul 19;10(7):e0004856. doi: 10.1371/journal.pntd.0004856. eCollection 2016 Jul.
In recent years, comparative genome sequence analysis of African Mycobacterium ulcerans strains isolated from Buruli ulcer (BU) lesion specimen has revealed a very limited genetic diversity of closely related isolates and a striking association between genotype and geographical origin of the patients. Here, we compared whole genome sequences of five M. ulcerans strains isolated in 2004 or 2013 from BU lesions of four residents of the Offin river valley with 48 strains isolated between 2002 and 2005 from BU lesions of individuals residing in the Densu river valley of Ghana. While all M. ulcerans isolates from the Densu river valley belonged to the same clonal complex, members of two distinct clonal complexes were found in the Offin river valley over space and time. The Offin strains were closely related to genotypes from either the Densu region or from the Asante Akim North district of Ghana. These results point towards an occasional involvement of a mobile reservoir in the transmission of M. ulcerans, enabling the spread of bacteria across different regions.
近年来，对从布氏溃疡（BU）病变标本中分离出的非洲溃疡分枝杆菌菌株进行的比较基因组序列分析显示，密切相关的分离株的遗传多样性非常有限，并且基因型与患者的地理来源之间存在显著关联。在此，我们比较了2004年或2013年从奥芬河谷四名居民的BU病变中分离出的五株溃疡分枝杆菌菌株的全基因组序列，以及2002年至2005年期间从加纳登苏河谷居民的BU病变中分离出的48株菌株的全基因组序列。虽然所有来自登苏河谷的溃疡分枝杆菌分离株都属于同一个克隆复合体，但在奥芬河谷的不同时空发现了两个不同克隆复合体的成员。奥芬菌株与来自登苏地区或加纳阿散蒂阿基姆北区的基因型密切相关。这些结果表明，移动储存库偶尔参与溃疡分枝杆菌的传播，使细菌能够在不同地区传播。
