Uda Ryoko M, Kato Yutaka, Takei Michiko
Department of Chemical Engineering, Nara National College of Technology, Yata 22, Yamato-koriyama, Nara 639-1080, Japan.
Department of Chemical Engineering, Nara National College of Technology, Yata 22, Yamato-koriyama, Nara 639-1080, Japan.
Colloids Surf B Biointerfaces. 2016 Oct 1;146:716-21. doi: 10.1016/j.colsurfb.2016.07.018. Epub 2016 Jul 7.
When working with liposomes analogous to cell membranes, it is important to develop substrates that can regulate interactions with the liposome surface in response to light. We achieved a photo-triggered release from liposomes by using a copolymer of poly(vinyl alcohol) carrying a malachite green moiety (PVAMG). Although PVAMG is a neutral polymer under dark conditions, it is photoionized upon exposure to UV light, resulting in the formation of a cationic site for binding to liposomes with a negatively charged surface. Under UV irradiation, PVAMG showed effective interaction with liposomes, releasing the encapsulated compound; however, this release was negligible under dark conditions. The poly(vinyl alcohol) moiety of PVAMG played an important role in the photo-triggered release. This release was caused by membrane destabilization without lipid solubilization. We also investigated different aspects of liposome/PVAMG interactions, including PVAMG-induced fusion between the liposomes and the change in the liposome morphologies.
在处理类似于细胞膜的脂质体时，开发能够响应光来调节与脂质体表面相互作用的底物非常重要。我们通过使用带有孔雀石绿部分的聚乙烯醇共聚物（PVAMG）实现了从脂质体的光触发释放。尽管PVAMG在黑暗条件下是一种中性聚合物，但在紫外光照射下会发生光离子化，从而形成一个阳离子位点，用于与带负电荷表面的脂质体结合。在紫外光照射下，PVAMG与脂质体表现出有效的相互作用，释放出包封的化合物；然而，在黑暗条件下这种释放可以忽略不计。PVAMG的聚乙烯醇部分在光触发释放中起重要作用。这种释放是由膜不稳定引起的，而没有脂质溶解。我们还研究了脂质体/PVAMG相互作用的不同方面，包括PVAMG诱导的脂质体之间的融合以及脂质体形态的变化。
