Department of Chemical Engineering, Nara National College of Technology, Yata 22, Yamato-koriyama, Nara 639-1080, Japan.
Langmuir. 2010 Apr 20;26(8):5444-50. doi: 10.1021/la904190c.
Photoinduced morphological changes in phosphatidylcholine vesicles are triggered by a Malachite Green leuconitrile derivative dissolved in the lipidic membrane, and are observed at Malachite Green derivative/lipid ratios <5 mol %. This Malachite Green derivative is a photoresponsive compound that undergoes ionization to afford a positive charge on the molecule by UV irradiation. The Malachite Green derivative exhibits amphiphilicity when ionized photochemically, whereas it behaves as a lipophilic compound under dark conditions. Cryo-transmission electron microscopy was used to determine vesicle morphology. The effects of the Malachite Green derivative on vesicles were studied by dynamic light scattering and fluorescence resonance energy transfer. Irradiation of vesicles containing the Malachite Green derivative induces nonspherical vesicle morphology, fusion of vesicles, and membrane solubilization, depending on conditions. Furthermore, irradiation of the Malachite Green derivative induces the release of a vesicle-encapsulated compound.
在脂质体中溶解的孔雀石绿亮甲腈衍生物引发的磷脂囊泡的光诱导形态变化，在孔雀石绿衍生物/脂质比 <5 mol % 时观察到。该孔雀石绿衍生物是一种光响应化合物，通过紫外线照射进行电离，从而在分子上提供正电荷。当孔雀石绿衍生物光化学电离时，它表现出两亲性，而在黑暗条件下则表现为亲脂性化合物。使用低温透射电子显微镜来确定囊泡形态。通过动态光散射和荧光共振能量转移研究了孔雀石绿衍生物对囊泡的影响。含有孔雀石绿衍生物的囊泡的辐照诱导非球形囊泡形态、囊泡融合和膜溶解，这取决于条件。此外，孔雀石绿衍生物的辐照诱导囊泡包封化合物的释放。
