为可重复的纳米光谱测量制备具有良好定义的 DNA 样品。

Preparation of Well-Defined DNA Samples for Reproducible Nanospectroscopic Measurements.

机构信息

Laboratory of Organic Chemistry, Department of Chemistry and Applied Biosciences, ETH Zurich, 8093, Zurich, Switzerland.

The Henryk Niewodniczanski Institute of Nuclear Physics, Polish Academy of Sciences, 31-342, Krakow, Poland.

出版信息

Small. 2016 Sep;12(35):4821-4829. doi: 10.1002/smll.201601711. Epub 2016 Jul 19.

DOI:10.1002/smll.201601711

PMID:27434680

Abstract

Due to its well-defined topology and chemical structure, DNA could become a biological standard sample in the field of nanospectroscopy. Tip-enhanced Raman spectroscopy (TERS) provides new insights into individual DNA molecules immobilized on flat mica crystals. The high sensitivity of TERS is used to assess the chemical changes that appear in DNA upon different surface immobilization protocols.

摘要

由于其明确的拓扑结构和化学结构，DNA 可能成为纳米光谱学领域的生物标准样品。尖端增强拉曼光谱（TERS）为固定在平坦云母晶体上的单个 DNA 分子提供了新的见解。TERS 的高灵敏度用于评估不同表面固定化方案下 DNA 出现的化学变化。