过氧化物酶-氧化酶反应中底物硫醇与非底物硫醇之间的协同作用。

Synergism between substrate and non-substrate thiols in peroxidase-oxidase reactions.

作者信息

Svensson B E

机构信息

Research and Development Laboratories, Södertälje, Sweden.

出版信息

Chem Biol Interact. 1989;70(3-4):323-37. doi: 10.1016/0009-2797(89)90053-7.

DOI:10.1016/0009-2797(89)90053-7

PMID:2743475

Abstract

Cysteamine and reduced glutathione were shown to act synergistically as peroxidase-oxidase substrates as measured by oxygen consumption and Nitro Blue Tetrazolium reduction. Cysteine methyl ester could be substituted for cysteamine and N-acetylcysteine and penicillamine could be substituted for glutathione. The involvement of reduced oxygen species and the effects of pH and chloride were studied. A possible mechanism of peroxidase-oxidase oxidation of cysteamine and glutathione is proposed. These studies show that peroxidase oxidase reactions can occur with close to physiological concentrations of peroxidase and thiols.

摘要

通过耗氧量和硝基蓝四唑还原法测定，半胱胺和还原型谷胱甘肽作为过氧化物酶-氧化酶底物表现出协同作用。半胱氨酸甲酯可替代半胱胺，N-乙酰半胱氨酸和青霉胺可替代谷胱甘肽。研究了活性氧的参与情况以及pH值和氯离子的影响。提出了半胱胺和谷胱甘肽过氧化物酶-氧化酶氧化的可能机制。这些研究表明，过氧化物酶氧化酶反应可以在接近生理浓度的过氧化物酶和硫醇存在下发生。