Masuzawa T, Sekiguchi T, Shimizu T, Iwamoto Y, Morita T, Mifuchi I, Yanagihara Y
Chem Pharm Bull (Tokyo). 1989 Feb;37(2):414-7. doi: 10.1248/cpb.37.414.
Nine cell lines producing monoclonal antibodies (MAbs) against Leptospira interrogans serovar canicola strain Moulton were established by the cell fusion technique. The immunological reactivity of these MAbs with various kinds of serogroups, serovars and strains were examined by microscopic agglutination test (MAT) and enzyme-linked immunosorbent assay (ELISA). MAbs W1-W3 derived from mice, which were immunized with whole cells of the strain Moulton, reacted with the serogroups Canicola, Icterohaemorrhagiae and Pyrogenes. On the other hand, MAbs A1-A6 derived from mice immunized with the outer envelope (OE) fraction, which showed a potent protective activity and was extracted with ammonium hydroxide from the strain Moulton, reacted specifically with the serogroup Canicola alone in MAT. A difference in antigenic structure between subserogroup A (canicola subgroup) and subserogroups B (schüeffneri subgroup) of the serogroup Canicola was demonstrated by MAT using the MAbs. All the MAbs clearly agglutinated serovars of subserogroup A except for serovars kamituga, jonsis and bindjei, but did not react with any serovars of subserogroup B. These findings suggest that MAb highly specific to each serovar is readily available by OE immunization and is useful for the classification of Leptospira.
通过细胞融合技术建立了9株产生抗犬钩端螺旋体莫尔顿菌株单克隆抗体（MAb）的细胞系。通过显微镜凝集试验（MAT）和酶联免疫吸附测定（ELISA）检测了这些单克隆抗体与各种血清群、血清型和菌株的免疫反应性。用莫尔顿菌株全细胞免疫的小鼠产生的单克隆抗体W1 - W3与犬血清群、出血性黄疸血清群和热原血清群发生反应。另一方面，用从莫尔顿菌株中用氢氧化铵提取的具有强保护活性的外膜（OE）组分免疫的小鼠产生的单克隆抗体A1 - A6在MAT中仅与犬血清群特异性反应。使用这些单克隆抗体的MAT证明了犬血清群的A亚血清群（犬亚群）和B亚血清群（舒夫纳里亚群）之间抗原结构的差异。所有单克隆抗体都能清楚地凝集A亚血清群的血清型，除了卡米图加血清型、琼西斯血清型和宾杰伊血清型，但不与B亚血清群的任何血清型发生反应。这些发现表明，通过OE免疫可以很容易地获得对每种血清型高度特异的单克隆抗体，并且对钩端螺旋体的分类很有用。
