伊朗东北部通过显微镜凝集试验检测钩端螺旋体抗体
Detection of leptospiral antibodies by microscopic agglutination test in north-east of Iran.
作者信息
Ehsanollah Sakhaee, Gholam Reza Abdollah Pour
机构信息
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Iran.
出版信息
Asian Pac J Trop Biomed. 2011 Jun;1(3):227-9. doi: 10.1016/S2221-1691(11)60032-4.
OBJECTIVE
To detect leptospiral antibodies by microscopic agglutination test (MAT) in north-east of Iran.
METHODS
This study was conducted to evaluate prevalence of human leptospiral infections by MAT, using six current reference strains of Leptospira interrogans in north-east of Iran. A total of 285 serum samples were collected from three north-east provinces of Iran, from December, 2009 to June, 2010.
RESULTS
Antibodies were detected at least against one serovar of Leptospira interrogans in 45 sera (15.79 %) among 285 samples at a dilution 1:100 or greater. Positive titers against more than one serovar were detected in 24 sera of the positive samples. Therefore, there were 75 positive reactions against different serovar of Leptospira interrogans. Positive titers were recorded against serovar icterohaemorrhagiae (31 samples), hardjo (26 samples), grippotyphosa (7 samples), pomona (5 samples), canicola (4 samples) and ballum (2 sample).
CONCLUSIONS
In present study the most prevalent (Leptospira icterohaemorrhagiae) and the least prevalent (Leptospira ballum) serovar are different from previous studies. Maybe, species and prevalence of serovars change during the time in one area and between regions.
目的
通过显微镜凝集试验（MAT）检测伊朗东北部人群的钩端螺旋体抗体。
方法
本研究采用6株问号钩端螺旋体现行参考菌株，通过MAT评估伊朗东北部人群钩端螺旋体感染的流行情况。2009年12月至2010年6月，从伊朗东北部三个省份共采集了285份血清样本。
结果
在285份样本中，45份血清（15.79%）在稀释度为1:100或更高时检测到至少针对一种问号钩端螺旋体血清型的抗体。在阳性样本的24份血清中检测到针对多种血清型的阳性滴度。因此，针对问号钩端螺旋体不同血清型共有75次阳性反应。记录到针对出血性黄疸型血清型（31份样本）、五日热型血清型（26份样本）、爪哇型血清型（7份样本）、波摩那型血清型（5份样本）、犬型血清型（4份样本）和拜伦型血清型（2份样本）的阳性滴度。
结论
在本研究中，最常见的血清型（出血性黄疸型钩端螺旋体）和最不常见的血清型（拜伦型钩端螺旋体）与先前的研究不同。也许，一个地区内以及不同地区间血清型的种类和流行率会随时间变化。
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