Hamann Fernanda Regina, Zago Adriana Maria, Rossato Mateus Fortes, Beck Veronica Rubert, Mello Carlos Fernando, de Brum Thiele Faccim, de Carvalho Leandro Machado, Faccin Henrique, Oliveira Sara Marchesan, Rubin Maribel Antonello
Graduate Program in Biological Sciences: Toxicological Biochemistry, Center of Natural and Exact Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Pharmacy Undergraduation Program, Centro Universitário Franciscano - UNIFRA, Santa Maria, RS, Brazil.
J Ethnopharmacol. 2016 Nov 4;192:210-216. doi: 10.1016/j.jep.2016.07.045. Epub 2016 Jul 17.
Vitex megapotamica (Spreng) Moldenke has been used in South American folk medicine to treat inflammatory diseases. However, the effects of V. megapotamica on animal models of nociception and depression have not been evaluated.
This study investigated whether the crude leaf extract of V. megapotamica exhibits antinociceptive and antidepressant-like effects in a Freund's adjuvant-induced chronic inflammation and depression model.
Chronic inflammation was induced in rats by the intraplantar administration of complete Freund's adjuvant (CFA; 100μl). The effect of oral crude extract of V. megapotamica (VmE; 3-30mg/kg, p.o.) on nociception (thermal hyperalgesia, mechanical allodynia and arthritis score), inflammation (edema, myeloperoxidase activity), immobility (forced swimming test), locomotor activity (open field), gastrointestinal transit, hyperalgesia and naloxone-precipitated morphine withdrawal syndrome was evaluated. Naloxone (0.4mg/kg, i.p.) was used to investigate the involvement of opioid system in the currently described effects of VmE.
Crude extract caused antinociceptive/antidepressant-like effects in the CFA-induced chronic inflammation model, which was prevented by naloxone. The VmE extract (10mg/kg, p.o.) did not alter the locomotor activity, gastrointestinal function and inflammatory parameters and did not cause hyperalgesia.
V. megapotamica induces opioid-dependent antinociception and antidepressant-like effect, without anti-inflammatory activity. The results support the use of VmE as analgesic and antidepressant.
大果牡荆（Vitex megapotamica (Spreng) Moldenke）已被用于南美民间医学治疗炎症性疾病。然而，大果牡荆对伤害感受和抑郁动物模型的影响尚未得到评估。
本研究调查了大果牡荆粗叶提取物在弗氏佐剂诱导的慢性炎症和抑郁模型中是否具有抗伤害感受和抗抑郁样作用。
通过足底注射完全弗氏佐剂（CFA；100μl）诱导大鼠慢性炎症。评估大果牡荆口服粗提取物（VmE；3 - 30mg/kg，口服）对伤害感受（热痛觉过敏、机械性异常性疼痛和关节炎评分）、炎症（水肿、髓过氧化物酶活性）、不动时间（强迫游泳试验）、运动活性（旷场试验）、胃肠转运、痛觉过敏和纳洛酮诱发的吗啡戒断综合征的影响。使用纳洛酮（0.4mg/kg，腹腔注射）研究阿片系统在VmE上述所描述作用中的参与情况。
粗提取物在CFA诱导的慢性炎症模型中产生了抗伤害感受/抗抑郁样作用，且该作用被纳洛酮阻断。VmE提取物（10mg/kg，口服）未改变运动活性、胃肠功能和炎症参数，也未引起痛觉过敏。
大果牡荆诱导阿片依赖的抗伤害感受和抗抑郁样作用，无抗炎活性。结果支持将VmE用作镇痛药和抗抑郁药。
