冷大气等离子体对红色毛癣菌生长、麦角固醇生物合成及角蛋白酶活性的抑制作用
Inhibitory effects of cold atmospheric plasma on the growth, ergosterol biosynthesis, and keratinase activity in Trichophyton rubrum.
作者信息
Shapourzadeh Atena, Rahimi-Verki Neda, Atyabi Seyed-Mohammad, Shams-Ghahfarokhi Masoomeh, Jahanshiri Zahra, Irani Shiva, Razzaghi-Abyaneh Mehdi
机构信息
Department of Mycology, Pasteur Institute of Iran, Tehran, 13164, Iran; Department of Biochemistry, Faculty of Basic Science, Islamic Azad University Damghan Branch, Damghan, Iran.
Department of Biochemistry, Faculty of Basic Science, Islamic Azad University Damghan Branch, Damghan, Iran.
出版信息
Arch Biochem Biophys. 2016 Oct 15;608:27-33. doi: 10.1016/j.abb.2016.07.012. Epub 2016 Jul 18.
BACKGROUND
Dermatophytosis is the most important superficial fungal infection which affects nearly 20% of human population worldwide. Recurrence of disease and emerging resistance of Trichophyton rubrum to synthetic antifungals are the main problems in control of dermatophytosis. The purpose of this study was to evaluate the effect of cold atmospheric plasma (CAP) on T. rubrum growth, ergosterol biosynthesis and keratinase activity.
METHODS
A CAP system, comprised of helium 98% - oxygen 2% (He/O2), was used. Trichophyton rubrum conidia suspensions were treated with CAP in time periods of 90, 120, 150 and 180 s in 96-well microplates. Fungal growth was evaluated by counting the colony forming unit (CFU). Fungal dry weight, ergosterol biosynthesis and keratinase activity were evaluated in CAP-treated T. rubrum and untreated controls.
RESULTS
T. rubrum growth was significantly inhibited by 62%-91%. CAP strongly suppressed fungal ergosterol biosynthesis by 27%-54%. The keratinase activity was increased by 7.30%-21.88% up to 120 s CAP exposure.
CONCLUSION
Our results demonstrated for the first time that CAP inhibits T. rubrum growth, suppresses ergosterol biosynthesis and increases moderately keratinase activity in a dose-dependent manner. Overall, CAP exposure could be a potentially useful method for treatment of clinical cases of human and animal dermatophytoses.
背景
皮肤癣菌病是最重要的浅表真菌感染，全球近20%的人口受其影响。疾病复发以及红色毛癣菌对合成抗真菌药物出现耐药性是控制皮肤癣菌病的主要问题。本研究的目的是评估冷大气等离子体（CAP）对红色毛癣菌生长、麦角甾醇生物合成和角蛋白酶活性的影响。
方法
使用由98%氦气-2%氧气（He/O2）组成的CAP系统。在96孔微孔板中，将红色毛癣菌分生孢子悬液分别用CAP处理90、120、150和180秒。通过计数菌落形成单位（CFU）评估真菌生长。对经CAP处理的红色毛癣菌和未处理的对照进行真菌干重、麦角甾醇生物合成和角蛋白酶活性评估。
结果
红色毛癣菌的生长受到显著抑制，抑制率为62%-91%。CAP强烈抑制真菌麦角甾醇生物合成，抑制率为27%-54%。在CAP暴露长达120秒时，角蛋白酶活性提高了7.30%-21.88%。
结论
我们的结果首次表明，CAP以剂量依赖的方式抑制红色毛癣菌生长，抑制麦角甾醇生物合成，并适度提高角蛋白酶活性。总体而言，暴露于CAP可能是治疗人和动物皮肤癣菌病临床病例的一种潜在有用方法。
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