Department of Environmental Engineering and Oral Biopathology Graduate Program, Institute of Science and Technology, São Paulo State University (UNESP), Avenida Engenheiro Francisco José Longo, 777, São José dos Campos, SP, 12245-000, Brazil.
Departament of Pharmacy, Taubaté Institute of Higher Education (ITES), Avenida Dom Pedro I, 3575, Taubaté, SP, 12090-000, Brazil.
Mycopathologia. 2019 Oct;184(5):585-595. doi: 10.1007/s11046-019-00375-2. Epub 2019 Aug 30.
This study aimed to evaluate the effects of cold atmospheric pressure plasma (CAPP) jet on Trichophyton rubrum growth, germination and adherence to nail. The effects of plasma jet on T. rubrum conidia germination and on mycelial growth were evaluated by in vitro assays. An ex vivo nail infection model was used to evaluate the effects on conidia adherence and infection. Biochemical analyses of nail fragments exposed or not to CAPP were performed by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. Plasma jet exposure for 10 and 15 min completely inhibited mycelial growth after only one exposure. Fifteen minutes of exposure could reduce conidia germination in suspension. Fungal suspensions exposed to plasma jet for 10 and 15 min were not able to infect nail specimens. These results were corroborated by ATR-FTIR analyses of nail fragments. In conclusion, single exposure to CAPP for 15 min was able to inhibit fungal growth, adherence and infection capacity. The results suggest that cold atmospheric plasma jet can be a promising alternative for the treatment of onychomycoses caused by T. rubrum.
本研究旨在评估冷大气压等离子体（CAPP）射流对红色毛癣菌生长、发芽和黏附指甲的影响。通过体外试验评估等离子射流对红色毛癣菌孢子发芽和菌丝生长的影响。使用体外指甲感染模型评估对孢子黏附和感染的影响。通过衰减全反射傅里叶变换红外（ATR-FTIR）光谱对暴露或未暴露于 CAPP 的指甲碎片进行生化分析。仅暴露 10 和 15 分钟即可完全抑制一次暴露后的菌丝生长。15 分钟的暴露可以减少悬浮液中的孢子发芽。暴露于等离子射流 10 和 15 分钟的真菌悬浮液无法感染指甲标本。ATR-FTIR 分析指甲碎片的结果证实了这一点。总之，单次暴露于 CAPP 15 分钟即可抑制真菌生长、黏附和感染能力。结果表明，冷大气压等离子体射流可能是治疗由红色毛癣菌引起的甲真菌病的有前途的替代方法。
